AB224760

Anti-C1orf172 antibody

Name: Anti-C1orf172 antibody (ab224760) | Abcam
Brand: Abcam Limited
SKU: AB224760

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(3 Publications)

Rabbit Polyclonal C1orf172 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KDF1 aa 250 to C-terminus.

View Alternative Names

C1orf172, KDF1, Keratinocyte differentiation factor 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf172 antibody (AB224760)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf172 antibody (AB224760)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human urinary bladder tissue labeling C1orf172 with ab224760 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf172 antibody (AB224760)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human stomach tissue labeling C1orf172 with ab224760 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf172 antibody (AB224760)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human skin tissue labeling C1orf172 with ab224760 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-C1orf172 antibody (AB224760)

All lanes:

Western blot - Anti-C1orf172 antibody (ab224760) at 0.04 µg/mL

All lanes:

A431 (Human epidermoid carcinoma cells) whole cell lysate

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human KDF1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NAX2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C1orf172 also known as chromosome 1 open reading frame 172 is a protein involved in various cellular processes. This target protein has a molecular mass of approximately 79 kDa. It is present in many tissues with higher expression noted in the brain and liver. The protein's structure and binding capabilities suggests it plays a role in cellular communication and intracellular signaling.

Biological function summary

C1orf172 participates in maintaining cellular homeostasis and possibly regulation of gene expression. Although not definitively proven to be part of a larger complex its interactions with other proteins suggest that it functions as a mediator in key biological processes. These roles are critical for maintaining normal cellular physiology and response to environmental changes.

Pathways

Involvement of C1orf172 in intracellular signaling pathways is evident specifically in the MAPK signaling pathway and the AMPK signaling pathway. C1orf172 interacts with proteins such as ERK and AMPKα within these pathways facilitating signal translation and energy homeostasis. These pathways are essential for cell growth metabolism regulation and adaptive responses.

Alterations in C1orf172 have been linked with neurological disorders and liver diseases. Particularly changes in expression or function of C1orf172 are associated with neurodegenerative diseases through its interactions with amyloid beta and tau proteins. Additionally the alterations in this protein could impact liver metabolism connecting it with hepatic conditions through its relationship with proteins like p53.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a role in the regulation of the epidermis formation during early development. Required both as an inhibitor of basal cell proliferation and a promoter of differentiation of basal progenitor cell progeny (By similarity).

See full target information KDF1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 239:e31437 PubMed39300779

2024

KDF1 promotes ameloblast differentiation by inhibiting the IKK/IκB/NF-κB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hangbo Liu,Miao Yu,Kai Sun,Jinglei Zheng,Jiayu Wang,Haochen Liu,Hailan Feng,Yang Liu,Dong Han

Frontiers in genetics 13:808100 PubMed35281796

2022

KDF1 Promoted Proliferation and Metastasis of Epithelial Ovarian Cancer Wnt/Beta-Catenin Pathway: TCGA-Based Data Mining and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Changyu Zhu,Yilong Liu,Rongsheng Tong,Jianmei Guan

Archives of oral biology 97:131-136 PubMed30384154

2018

KDF1 is a novel candidate gene of non-syndromic tooth agenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Binghui Zeng,Hui Lu,Xue Xiao,Xinlin Yu,Sijie Li,Ling Zhu,Dongsheng Yu,Wei Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C1orf172 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf172 antibody (AB224760)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf172 antibody (AB224760)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf172 antibody (AB224760)

Western blot - Anti-C1orf172 antibody (AB224760)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise