JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250166

Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal C1orf69/IBA57 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C1orf69, IBA57, Iron-sulfur cluster assembly factor homolog

2 Images
Flow Cytometry (Intracellular) - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (AB250166)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (AB250166)

This data was developed using ab180161, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Raji cells labelling C1orf69/IBA57 using ab180161 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (AB250166)
  • WB

Supplier Data

Western blot - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (AB250166)

This data was developed using ab180161, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C1orf69/IBA57 antibody [EPR11758(2)] (<a href='/en-us/products/primary-antibodies/c1orf69-iba57-antibody-epr117582-ab180161'>ab180161</a>) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

Caco 2 cell lysate at 10 µg

Lane 5:

293T cell lysate at 10 µg

Lane 6:

HepG2 cell lysate at 10 µg

Lane 7:

Human fetal liver lysate at 10 µg

Predicted band size: 38 kDa

true

  • Unconjugated

    Anti-C1orf69/IBA57 antibody [EPR11758(2)]

  • 578 PE

    PE Anti-C1orf69 antibody [EPR11758(2)]

  • 660 APC

    APC Anti-C1orf69 antibody [EPR11758(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-C1orf69 antibody [EPR11758(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-C1orf69 antibody [EPR11758(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-C1orf69 antibody [EPR11758(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-C1orf69 antibody [EPR11758(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-C1orf69/IBA57 antibody [EPR11758(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11758(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250166 is the carrier-free version of ab180161.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway.
See full target information IBA57
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB58724

Anti-Lipoic Acid antibody

  • 0
  • 0
Assay Kits

AB109908

Complex II Activity Assay Kit (Colorimetric)

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)

  • 5
  • 2
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74
Primary Antibodies

AB133579

Anti-PSMA antibody [EPR6253]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA antibody [EPR6253] (AB133579)

  • 3
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com