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Rabbit Recombinant Monoclonal C1orf69/IBA57 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (AB250166), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (AB250166), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Version

Target data

Function

Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway.

Alternative names

C1orf69, IBA57, Iron-sulfur cluster assembly factor homolog

Recommended products

Rabbit Recombinant Monoclonal C1orf69/IBA57 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR11758(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250166 is the carrier-free version of Anti-C1orf69/IBA57 antibody [EPR11758(2)] ab180161.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (ab250166), expandable thumbnail

    Western blot - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (ab250166)

    This data was developed using Anti-C1orf69/IBA57 antibody [EPR11758(2)] ab180161, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-C1orf69/IBA57 antibody [EPR11758(2)] (Anti-C1orf69/IBA57 antibody [EPR11758(2)] ab180161) at 1/1000 dilution

    Lane 1: Human fetal heart lysate at 10 µg

    Lane 2: Raji cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: Caco 2 cell lysate at 10 µg

    Lane 5: 293T cell lysate at 10 µg

    Lane 6: HepG2 cell lysate at 10 µg

    Lane 7: Human fetal liver lysate at 10 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa

  • Flow Cytometry (Intracellular) - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (ab250166), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-C1orf69/IBA57 antibody [EPR11758(2)] - BSA and Azide free (ab250166)

    This data was developed using Anti-C1orf69/IBA57 antibody [EPR11758(2)] ab180161, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of permeabilized Raji cells labelling C1orf69/IBA57 using Anti-C1orf69/IBA57 antibody [EPR11758(2)] ab180161 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com