Anti-C1orf93 antibody [EPR13703] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-C1orf93 antibody [EPR13703] - BSA and Azide free (AB250265)

This data was developed using ab180932, the same antibody clone in a different buffer formulation.

Immunoprecipitation of fetal kidney cell line labeling C1orf93 using ab180932 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-C1orf93 antibody [EPR13703] (<a href='/en-us/products/primary-antibodies/c1orf93-antibody-epr13703-ab180932'>ab180932</a>) at 1/60 dilution

Lane 1:

fetal kidney cell line at 10 µg

Lane 2:

Negative control

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

Predicted band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-C1orf93 antibody [EPR13703] - BSA and Azide free (AB250265)

This data was developed using ab180932, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C1orf93 antibody [EPR13703] (<a href='/en-us/products/primary-antibodies/c1orf93-antibody-epr13703-ab180932'>ab180932</a>) at 1/1000 dilution

All lanes:

THP1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-C1orf93 antibody [EPR13703] - BSA and Azide free (AB250265)

This data was developed using ab180932, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C1orf93 antibody [EPR13703] (<a href='/en-us/products/primary-antibodies/c1orf93-antibody-epr13703-ab180932'>ab180932</a>) at 1/5000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Predicted band size: 21 kDa

false