AB182451

Anti-C1q antibody [4.8]

Name: Anti-C1q antibody [4.8] KO tested (ab182451) | Abcam
Brand: Abcam Limited
SKU: AB182451
Price: 330 USD
Availability: InStock
Rating: 4 (6 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(6 Reviews)

(100 Publications)

Anti-C1q antibody [4.8] (ab182451) is a rabbit monoclonal antibody detecting C1q in IHC-Fr. Suitable for Mouse.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications

View Alternative Names

Complement C1q subcomponent subunit A, C1qa

2 Images

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] (AB182451)

IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] (AB182451)

ab182451 (1/500) staining C1q in 15μm coronal brain sections from postnatal day 90 C1qA KO mice and WT litter-mates (Botto et al., 1998) by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.3% Triton. An Alexa Flour-conjugated Goat anti-Mouse IgG polyclonal (1/500) was used as the secondary antibody. Positive staining on wild type (WT) mouse cerebral cortex, and no staining on knockout (KO) mouse cerebral cortex.

This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital.

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] (AB182451)

Immunohistochemical analysis of frozen mouse spleen labelling C1q with ab182451 at a concentration of 0.1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a ChromoMap DAB kit and anti-rabbit HQ and anti-HQ HRP detection. ab182451 Anti-C1q antibody [4.8] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Carrier free

Anti-C1q antibody [4.8] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

4.8

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Anti-C1q antibody [4.8] (ab182451) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-Fr in mouse samples.

Anti-C1q antibody [4.8] (ab182451) has been cited over 67 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-C1q antibody [4.8] (ab182451) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

The specificity of Anti-C1q antibody [4.8] (ab182451) has been confirmed by testing in knockout samples.

Anti-C1q antibody [4.8] (ab182451) specifically detects C1q (UniProt ID: P02745; Molecular weight: 24kDa) and is sold in 100 µL and 1 mL selling sizes.

This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University:

Alexander H. Stephan et al. , A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

See full target information C1qa

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Publications (100)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8694 PubMed41027899

2025

Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Wu,Ziyi Wang,Katherine R Croce,Fang Li,Jian Cui,Vivette D D'Agati,Rajesh K Soni,Ira Tabas,Ai Yamamoto,Hanrui Zhang

Journal of neuroinflammation 22:217 PubMed41024113

2025

Cholecystokinin ameliorates cognitive impairment via inhibiting microglia phagocytosis of excitatory synapses in sepsis-associated encephalopathy mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Chen,Zhen Li,Yubo Gao,Guangxi Piao,Yitong Li,Jingshu Hong,Qian Wang,Kaixi Liu,Jie Wang,Ailian Du,Luhua Chen,Xiangyang Guo,Zhengqian Li,Taotao Liu

Nature communications 16:7338 PubMed40781079

2025

Early Locus Coeruleus noradrenergic axon loss drives olfactory dysfunction in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Carolin Meyer,Theresa Niedermeier,Paul L C Feyen,Felix L Strübing,Boris-Stephan Rauchmann,Katerina Karali,Johanna Gentz,Yannik E Tillmann,Nicolas F Landgraf,Svenja-Lotta Rumpf,Katharina Ochs,Karin Wind-Mark,Gloria Biechele,Jessica Wagner,Selim Guersel,Carolin I Kurz,Meike Schweiger,Danilo Prtvar,Yuan Shi,Richard B Banati,Guo-Jun Liu,Ryan J Middleton,Gerda Mitteregger-Kretzschmar,Robert Perneczky,Thomas Koeglsperger,Jonas J Neher,Sabina Tahirovic,Matthias Brendel,Jochen Herms,Lars Paeger

Journal of neuroinflammation 22:138 PubMed40410792

2025

Diverse cell types establish a pathogenic immune environment in peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Choi,Amy Strickland,Hui Qi Loo,Wendy Dong,Lilianne Barbar,A Joseph Bloom,Yo Sasaki,Sheng Chih Jin,Aaron DiAntonio,Jeffrey Milbrandt

PloS one 20:e0323513 PubMed40373024

2025

Microglia remodeling in the visual thalamus of the DBA/2J mouse model of glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Thompson,Shaylah McCool,Jennie C Smith,Victoria Schaal,Gurudutt Pendyala,Sowmya Yelamanchili,Matthew J Van Hook

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 20:37 PubMed40227512

2025

Experimental autoimmune encephalomyelitis pathogenesis alters along animal age: impact of S100B expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Rita Ribeiro,Raquel Pereira,Catarina Barros,Andreia Barateiro,Ainhoa Alberro,Afonso P Basto,Luís Graça,Maria Vaz Pinto,Fábio M F Santos,Pedro M P Gois,Susan E Howlett,Adelaide Fernandes

iScience 28:112201 PubMed40212592

2025

Microglia target synaptic sites early during excitatory circuit disassembly in neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Alfred Yu,Camille Fang,Li Xuan Tan,Aparna Lakkaraju,Luca Della Santina,Yvonne Ou

Journal of neuroinflammation 22:68 PubMed40055725

2025

Microglial progranulin differently regulates hypothalamic lysosomal function in lean and obese conditions via cleavage-dependent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Chae Beom Park,Chan Hee Lee,Gil Myoung Kang,Se Hee Min,Min-Seon Kim

Nature communications 16:918 PubMed39843445

2025

Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Megumi Andoh,Natsuki Shinoda,Yusuke Taira,Tasuku Araki,Yuka Kasahara,Haruki Takeuchi,Masayuki Miura,Yuji Ikegaya,Ryuta Koyama

Communications biology 8:70 PubMed39820760

2025

An atlas of neuropathic pain-associated molecular pathological characteristics in the mouse spinal cord.

Applications

Unspecified application

Species

Unspecified reactive species

Fu-Lu Dong,Lina Yu,Pei-Da Feng,Jin-Xuan Ren,Xue-Hui Bai,Jia-Qi Lin,De-Li Cao,Yu-Tao Deng,Yan Zhang,Hui-Hui Shen,Hao Gong,Wen-Xing Sun,Dong-Qiu Chi,Yixiao Mei,Longfei Ma,Ming-Zhe Yin,Meng-Na Li,Peng-Fei Zhang,Nan Hu,Bing-Lin Zhou,Ying Liu,Xuan-Jie Zheng,Yi-Fan Chen,Da Zhong,Yuan-Xiang Tao,Min Yan,Bao-Chun Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C1q antibody [4.8]

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] (AB182451)

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] (AB182451)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (100)

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