AB227072

Anti-C1q antibody [4.8] - Low endotoxin, Azide free

RabMAb
Recombinant
KO Validated
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(7 Publications)

Rabbit Recombinant Monoclonal C1QA antibody. Carrier free. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 7 publications.

View Alternative Names

Complement C1q subcomponent subunit A, C1qa

1 Images

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] - Low endotoxin, Azide free (AB227072)

IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] - Low endotoxin, Azide free (AB227072)

Immunohistochemistry (Frozen sections) analysis of 15 μm coronal brain sections from postnatal day 90 C1qA knockout mice and wild-type litter-mates (Botto et al., 1998) labeling C1q with ab227072 at 1/500 dilution. Tissues fixed with 4% paraformaldehyde, and permeabilized using 0.3% triton. Goat anti-Mouse IgG H&L (Alexa Fluor^® 594) at 1/500 dilution was used as the secondary antibody (red). Nuclei counterstained with DAPI (teal).

This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital.

Unconjugated

Anti-C1q antibody [4.8]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

4.8

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

ab227072 is the carrier-free version of ab182451.

This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University: Alexander H. Stephan et al., A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

See full target information C1qa

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:65 PubMed40468412

2025

The UNC5C T835M mutation associated with Alzheimer's disease leads to neurodegeneration involving oxidative stress and hippocampal atrophy in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Devi Krishna Priya Karunakaran,Makenna Ley,Joanna Guo,Ammaarah Khatri,Katherine Sadleir,Jelena Popovic,Arun Kumar Upadhyay,Jeffrey Savas,Daniele Procissi,Jasvinder Atwal,Robert Vassar

Neuron 109:2275-2291.e8 PubMed34133945

2021

C9orf72 deficiency promotes microglial-mediated synaptic loss in aging and amyloid accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Deepti Lall,Ileana Lorenzini,Thomas A Mota,Shaughn Bell,Thomas E Mahan,Jason D Ulrich,Hayk Davtyan,Jessica E Rexach,A K M Ghulam Muhammad,Oksana Shelest,Jesse Landeros,Michael Vazquez,Junwon Kim,Layla Ghaffari,Jacqueline Gire O'Rourke,Daniel H Geschwind,Mathew Blurton-Jones,David M Holtzman,Rita Sattler,Robert H Baloh

Proceedings of the National Academy of Sciences of 113:10186-91 PubMed27559087

2016

Novel allele-dependent role for APOE in controlling the rate of synapse pruning by astrocytes.

Applications

IHC-P

Species

Mouse

Won-Suk Chung,Philip B Verghese,Chandrani Chakraborty,Julia Joung,Bradley T Hyman,Jason D Ulrich,David M Holtzman,Ben A Barres

Cell 165:921-35 PubMed27114033

2016

Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation.

Applications

ICC/IF

Species

Mouse

Hansen Lui,Jiasheng Zhang,Stefanie R Makinson,Michelle K Cahill,Kevin W Kelley,Hsin-Yi Huang,Yulei Shang,Michael C Oldham,Lauren Herl Martens,Fuying Gao,Giovanni Coppola,Steven A Sloan,Christine L Hsieh,Charles C Kim,Eileen H Bigio,Sandra Weintraub,Marek-Marsel Mesulam,Rosa Rademakers,Ian R Mackenzie,William W Seeley,Anna Karydas,Bruce L Miller,Barbara Borroni,Roberta Ghidoni,Robert V Farese,Jeanne T Paz,Ben A Barres,Eric J Huang

Science (New York, N.Y.) 352:712-716 PubMed27033548

2016

Complement and microglia mediate early synapse loss in Alzheimer mouse models.

Applications

IHC

Species

Mouse

Soyon Hong,Victoria F Beja-Glasser,Bianca M Nfonoyim,Arnaud Frouin,Shaomin Li,Saranya Ramakrishnan,Katherine M Merry,Qiaoqiao Shi,Arnon Rosenthal,Ben A Barres,Cynthia A Lemere,Dennis J Selkoe,Beth Stevens

Molecular neurodegeneration 11:24 PubMed27008854

2016

C1q propagates microglial activation and neurodegeneration in the visual axis following retinal ischemia/reperfusion injury.

Applications

IHC-Fr

Species

Mouse

Sean M Silverman,Byung-Jin Kim,Garreth R Howell,Joselyn Miller,Simon W M John,Robert J Wordinger,Abbot F Clark

The Journal of neuroscience : the official journal of the Society for Neuroscience 33:13460-74 PubMed23946404

2013

A dramatic increase of C1q protein in the CNS during normal aging.

Applications

IHC-FoFr

Species

Mouse

Alexander H Stephan,Daniel V Madison,José María Mateos,Deborah A Fraser,Emilie A Lovelett,Laurence Coutellier,Leo Kim,Hui-Hsin Tsai,Eric J Huang,David H Rowitch,Dominic S Berns,Andrea J Tenner,Mehrdad Shamloo,Ben A Barres

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C1q antibody [4.8] - Low endotoxin, Azide free

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] - Low endotoxin, Azide free (AB227072)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (7)

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