Rabbit Recombinant Monoclonal C1QA antibody. Carrier free. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 6 publications.
Images
![Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] - Low endotoxin, Azide free (AB227072), expandable thumbnail](https://content.abcam.com/products/images/c1q-antibody-48-low-endotoxin-azide-free-ab227072--immunohistochemistry-frozen-sections-img48915.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] - Low endotoxin, Azide free (AB227072)")
Publications
Filter by
- Neuron 109:2275-2291.e82021C9orf72 deficiency promotes microglial-mediated synaptic loss in aging and amyloid accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepti Lall,Ileana Lorenzini,Thomas A Mota,Shaughn Bell,Thomas E Mahan,Jason D Ulrich,Hayk Davtyan,Jessica E Rexach,A K M Ghulam Muhammad,Oksana Shelest,Jesse Landeros,Michael Vazquez,Junwon Kim,Layla Ghaffari,Jacqueline Gire O'Rourke,Daniel H Geschwind,Mathew Blurton-Jones,David M Holtzman,Rita Sattler,Robert H BalohPubMed 34133945
- Proceedings of the National Academy of Sciences of 113:10186-912016Novel allele-dependent role for APOE in controlling the rate of synapse pruning by astrocytes.
- Cell 165:921-352016Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation.
- Science (New York, N.Y.) 352:712-7162016Complement and microglia mediate early synapse loss in Alzheimer mouse models.
- Molecular neurodegeneration 11:242016C1q propagates microglial activation and neurodegeneration in the visual axis following retinal ischemia/reperfusion injury.
- The Journal of neuroscience : the official journal of the Society for Neuroscience 33:13460-742013A dramatic increase of C1q protein in the CNS during normal aging.Applications:IHC-FoFrReactive species:MouseAlexander H Stephan,Daniel V Madison,José María Mateos,Deborah A Fraser,Emilie A Lovelett,Laurence Coutellier,Leo Kim,Hui-Hsin Tsai,Eric J Huang,David H Rowitch,Dominic S Berns,Andrea J Tenner,Mehrdad Shamloo,Ben A BarresPubMed 23946404
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | |
|---|---|
| Mouse | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Target data
Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Alternative names
Complement C1q subcomponent subunit A, C1qa
Recommended products
Rabbit Recombinant Monoclonal C1QA antibody. Carrier free. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 4.8
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227072 is the carrier-free version of Anti-C1q antibody [4.8] ab182451.
This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University: Alexander H. Stephan et al., A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] - Low endotoxin, Azide free (ab227072), expandable thumbnail](https://content.abcam.com/products/images/c1q-antibody-48-low-endotoxin-azide-free-ab227072--immunohistochemistry-frozen-sections-img48915.jpg "Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] - Low endotoxin, Azide free (ab227072)")
This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital.Immunohistochemistry (Frozen sections) - Anti-C1q antibody [4.8] - Low endotoxin, Azide free (ab227072)
Immunohistochemistry (Frozen sections) analysis of 15 μm coronal brain sections from postnatal day 90 C1qA knockout mice and wild-type litter-mates (Botto et al., 1998) labeling C1q with ab227072 at 1/500 dilution. Tissues fixed with 4% paraformaldehyde, and permeabilized using 0.3% triton. Goat anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/500 dilution was used as the secondary antibody (red). Nuclei counterstained with DAPI (teal).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com