AB11861

Anti-C1q antibody [7H8]

Name: Anti-C1q antibody [7H8] (ab11861) | Abcam
Brand: Abcam Limited
SKU: AB11861
Price: 390 USD
Availability: InStock
Rating: 4 (9 reviews)

Recombinant
What is this?

(9 Reviews)

(28 Publications)

Anti-C1q antibody [7H8] (ab11861) is a rat monoclonal antibody detecting C1q in IHC-Fr. Suitable for Mouse.

- Over 10 publications
- Trusted since 2004

View Alternative Names

Complement C1q subcomponent subunit A, C1QA

1 Images

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (AB11861)

IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (AB11861)

Positive staining on P90 Wild-type mouse, and no staining on P90 C1qa Knockout Mouse . Primary antibody was applied at a dilution of 1/500 and anti-VGLUT1 antibody. Secondary antibodies including goat anti rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor 594 and goat anti guinea pig IgG (H+L) highly cross-adsorbed Alexa Fluor 488 were also employed at a dilution of 1/500.

Carrier free

Anti-C1q antibody [7H8] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7H8

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

IHC-Fr

applications

Specificity

Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-C1q antibody [7H8] (ab11861) is a rat recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr) in Human, Mouse samples.

Trusted by the scientific community
Anti-C1q [7H8] (ab11861) was first used in a scientific publication in 2004 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists
Anti-C1q [7H8] (ab11861) has over 5 independent reviews from customers.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12847249, PubMed : 19006321, PubMed : 24626930, PubMed : 29449492, PubMed : 3258649, PubMed : 34155115, PubMed : 6249812, PubMed : 6776418). The classical complement pathway is initiated by the C1Q subcomplex of the C1 complex, which specifically binds IgG or IgM immunoglobulins complexed with antigens, forming antigen-antibody complexes on the surface of pathogens : C1QA, together with C1QB and C1QC, specifically recognizes and binds the Fc regions of IgG or IgM via its C1q domain (PubMed : 12847249, PubMed : 19006321, PubMed : 24626930, PubMed : 29449492, PubMed : 3258649, PubMed : 6776418). Immunoglobulin-binding activates the proenzyme C1R, which cleaves C1S, initiating the proteolytic cascade of the complement system (PubMed : 29449492). The C1Q subcomplex is activated by a hexamer of IgG complexed with antigens, while it is activated by a pentameric IgM (PubMed : 19706439, PubMed : 24626930, PubMed : 29449492). The C1Q subcomplex also recognizes and binds phosphatidylserine exposed on the surface of cells undergoing programmed cell death, possibly promoting activation of the complement system (PubMed : 18250442).

See full target information C1QA

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:69 PubMed40055743

2025

Intravitreal delivery of NMO-IgG causes primary retinal damage in the absence of optic nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Biyue Chen,Huanfen Zhou,Mingming Sun,Wanqun Yang,Qianqian Li,Kaishu Yang,Honglu Song,Quangang Xu,Xintong Xu,Yuyu Li,Yanyan Yu,Shihui Wei,Tingjun Chen

Journal of neuroinflammation 21:246 PubMed39342308

2024

Exercise training upregulates CD55 to suppress complement-mediated synaptic phagocytosis in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hongkai Yao,Weifang Tong,Yunping Song,Ruoyu Li,Xuerui Xiang,Wen Cheng,Yunjiao Zhou,Yijing He,Yi Yang,Yunxi Liu,Siguang Li,Lingjing Jin

Journal of neuroinflammation 21:200 PubMed39129007

2024

The dopamine analogue CA140 alleviates AD pathology, neuroinflammation, and rescues synaptic/cognitive functions by modulating DRD1 signaling or directly binding to Abeta.

Applications

Unspecified application

Species

Unspecified reactive species

Sehyun Chae,Hyun-Ju Lee,Ha-Eun Lee,Jieun Kim,Yoo Joo Jeong,Yuxi Lin,Hye Yun Kim,Geoffray Leriche,Rachel S Ehrlich,Sascha Castro Lingl,Min-Duk Seo,Young-Ho Lee,Jerry Yang,Jae-Ick Kim,Hyang-Sook Hoe

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403064 PubMed39088351

2024

Clec7a Worsens Long-Term Outcomes after Ischemic Stroke by Aggravating Microglia-Mediated Synapse Elimination.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxi Wan,Mengfan He,Chun Cheng,Kexin Yang,Huanghui Wu,Peilin Cong,Xinwei Huang,Qian Zhang,Yufei Shi,Ji Hu,Li Tian,Lize Xiong

Glia 72:1874-1892 PubMed38946065

2024

Integrity of neural extracellular matrix is required for microglia-mediated synaptic remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Cangalaya,Weilun Sun,Stoyan Stoyanov,Ildiko Rita Dunay,Alexander Dityatev

mAbs 16:2297451 PubMed38170638

2024

Therapeutic efficacy of a potent anti-Venezuelan equine encephalitis virus antibody is contingent on Fc effector function.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer L Schwedler,Maxwell A Stefan,Christine E Thatcher,Peter R McIlroy,Anupama Sinha,Ashlee M Phillips,Christopher A Sumner,Colleen M Courtney,Christina Y Kim,Dina R Weilhammer,Brooke Harmon

Stem cell research & therapy 14:257 PubMed37726853

2023

Umbilical cord mesenchymal stem cell-derived apoptotic extracellular vesicles ameliorate cutaneous wound healing in type 2 diabetic mice via macrophage pyroptosis inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Wang,Lin Jing,Xiao Lei,Zhen Ma,Bei Li,Yuanyuan Shi,Wuyang Zhang,Yuan Li,Hongzhi Zhou,Kaijin Hu,Yang Xue,Yan Jin

Frontiers in molecular neuroscience 16:1191324 PubMed37415834

2023

Innate immune activation and aberrant function in the R6/2 mouse model and Huntington's disease iPSC-derived microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Gasser,Gaelle Gillet,Jorge S Valadas,Laura Rouvière,Apoorva Kotian,Wenqiang Fan,James Keaney,Irena Kadiu

Aging cell 22:e13860 PubMed37177836

2023

Absent in melanoma 2 mediates aging-related cognitive dysfunction by acting on complement-dependent microglial phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Ye,Shu Shu,Junqiu Jia,Min Sun,Siyi Xu,Xinyu Bao,Huijie Bian,Yi Liu,Meijuan Zhang,Xiaolei Zhu,Feng Bai,Yun Xu

Nature communications 14:2476 PubMed37120617

2023

Single-cell RNA sequencing reveals the fragility of male spermatogenic cells to Zika virus-induced complement activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Li-Bo Liu,Feng-Liang Liu,Yan-Hua Wu,Zi-Da Zhen,Dong-Ying Fan,Zi-Yang Sheng,Zheng-Ran Song,Jia-Tong Chang,Yong-Tang Zheng,Jing An,Pei-Gang Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C1q antibody [7H8]

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] (AB11861)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (28)

Product promise