JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB255973

Anti-C1q antibody [7H8] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rat Recombinant Monoclonal C1QA antibody. Carrier free. Suitable for IHC-Fr, ELISA, IHC-FoFr, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

Complement C1q subcomponent subunit A, C1qa

1 Images
Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] - BSA and Azide free (AB255973)
  • IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-C1q antibody [7H8] - BSA and Azide free (AB255973)

This data was developed using ab11861 the same antibody clone in a different buffer formulation.

Positive staining on P90 Wild-type mouse, and no staining on P90 C1qa Knockout Mouse . Primary antibody was applied at a dilution of 1/500 and anti-VGLUT1 antibody. Secondary antibodies including goat anti rabbit IgG (H+L) highly cross-adsorbed Alexa Fluor 594 and goat anti guinea pig IgG (H+L) highly cross-adsorbed Alexa Fluor 488 were also employed at a dilution of 1/500.

This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7H8

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-FoFr, ELISA, IHC-Fr

applications

Specificity

Immunization schedule and immunogen used has been described in the following article: Wasiliu, M et al; Monoclonal antibodies to complement components without the need of their prior purification. I. Antibodies to mouse C1q. Hybridoma 1989, 8 (6): 615.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab255973 is the carrier-free version of ab11861.

This product was switched from a hybridoma to a recombinant production format on 25th October 2021.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
See full target information C1qa
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e70166 PubMed39699038

2024

STING Driving Synaptic Phagocytosis of Hippocampal Microglia/Macrophages Contributes to Cognitive Impairment in Sepsis-Associated Encephalopathy in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Lv,Min Jia,Xiao Feng,Jia-Xiong Jian,Jian-Jun Yang,Da-Qing Ma,Mu-Huo Ji,Yu-Gang Diao,Jin-Chun Shen
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB170246

Human Complement C1q ELISA Kit

Sandwich ELISA - Human Complement C1q ELISA Kit (AB170246)

  • 0
  • 0
Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-C3 antibody [EPR19394] (AB200999)

  • 4
  • 9
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 49
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com