Rabbit Recombinant Monoclonal C1QB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--western-blot-img152329.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92747.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100604.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123816.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112716.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Alternative names
Complement C1q subcomponent subunit B, C1QB
Recommended products
Rabbit Recombinant Monoclonal C1QB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2981
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247575 is the carrier-free version of Anti-C1QB antibody [EPR2981] ab92508.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C1QB or complement component 1 q subcomponent beta chain is a protein that plays a mechanical role in the complement system. It has a mass of approximately 25 kDa and is expressed in many tissues including the liver and blood plasma. C1QB is a subunit of the C1q complex which is the first component of the classical complement pathway. This protein works with C1q's other subunits to recognize and bind to the Fc region of antibodies facilitating the activation of the complement pathway.
- Biological function summary
C1QB is involved in the immune response and helps in the clearance of pathogens and apoptotic cells. It forms part of the C1q complex which includes two other components: C1QA and C1QC. This complex binds to antibodies that are attached to antigens on the surface of pathogens activating the C1r and C1s serine proteases. The activated enzymes then cleave other complement proteins triggering a cascade that results in opsonization inflammation and cell lysis.
- Pathways
C1QB takes a significant role in the classical complement pathway and humoral immunity. The classical pathway starts with the binding of the C1 complex to antigen-antibody complexes. Through this mechanism C1QB associates with proteins such as C2 and C4 which further propagate the complement activation cascade. The classical complement pathway links to the lectin pathway where mannose-binding lectin substitutes for C1q to recognize pathogens.
- Associated diseases and disorders
Defects or deficiencies in C1QB relate to systemic lupus erythematosus (SLE) and hereditary angioedema. In SLE impaired function of C1QB leads to decreased clearance of immune complexes contributing to autoimmune reactions. This protein interacts with C1QA in disease mechanisms. In hereditary angioedema although C1QB is not directly defective its involvement in complement activation relates to mutations in C1 inhibitor protein. These mutations result in uncontrolled complement activation underlying the pathophysiology of the disorder.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--western-blot-img152329.jpg "Western blot - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
Western blot - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)
This data was developed using Anti-C1QB antibody [EPR2981] ab92508, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-C1QB antibody [EPR2981] (Anti-C1QB antibody [EPR2981] ab92508) at 1/1000 dilution
All lanes: Human plasma cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92747.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)
This data was developed using Anti-C1QB antibody [EPR2981] ab92508, the same antibody clone in a different buffer formulation.Anti-C1QB antibody [EPR2981] ab92508, at a 1/50 dilution, staining C1QB in paraffin embedded Human kidney tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100604.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)
This data was developed using Anti-C1QB antibody [EPR2981] ab92508, the same antibody clone in a different buffer formulation.Anti-C1QB antibody [EPR2981] ab92508 showing positive staining in Normal brain tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123816.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)
This data was developed using Anti-C1QB antibody [EPR2981] ab92508, the same antibody clone in a different buffer formulation.Anti-C1QB antibody [EPR2981] ab92508 showing positive staining in Normal breast tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112716.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)
This data was developed using Anti-C1QB antibody [EPR2981] ab92508, the same antibody clone in a different buffer formulation.Anti-C1QB antibody [EPR2981] ab92508 showing positive staining in Normal stomach tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123144.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)
This data was developed using Anti-C1QB antibody [EPR2981] ab92508, the same antibody clone in a different buffer formulation.Anti-C1QB antibody [EPR2981] ab92508 showing positive staining in Normal liver tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112763.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)
This data was developed using Anti-C1QB antibody [EPR2981] ab92508, the same antibody clone in a different buffer formulation.Anti-C1QB antibody [EPR2981] ab92508 showing positive staining in Normal spleen tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123144.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575), expandable thumbnail](https://content.abcam.com/products/images/c1qb-antibody-epr2981-bsa-and-azide-free-ab247575--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112763.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (ab247575)")
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