AB247575

Anti-C1QB antibody [EPR2981] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal C1QB antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

Complement C1q subcomponent subunit B, C1QB

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

This data was developed using ab92508, the same antibody clone in a different buffer formulation.

ab92508 showing positive staining in Normal breast tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

This data was developed using ab92508, the same antibody clone in a different buffer formulation.

ab92508, at a 1/50 dilution, staining C1QB in paraffin embedded Human kidney tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unknown

Western blot - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

This data was developed using ab92508, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C1QB antibody [EPR2981] (<a href='/en-us/products/primary-antibodies/c1qb-antibody-epr2981-ab92508'>ab92508</a>) at 1/1000 dilution

All lanes:

Human plasma cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 27 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

This data was developed using ab92508, the same antibody clone in a different buffer formulation.

ab92508 showing positive staining in Normal liver tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

This data was developed using ab92508, the same antibody clone in a different buffer formulation.

ab92508 showing positive staining in Normal brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

This data was developed using ab92508, the same antibody clone in a different buffer formulation.

ab92508 showing positive staining in Normal spleen tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

This data was developed using ab92508, the same antibody clone in a different buffer formulation.

ab92508 showing positive staining in Normal stomach tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Unconjugated

Anti-C1QB antibody [EPR2981]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2981

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "The use of an HRP/AP polymerized secondary antibody is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab247575 is the carrier-free version of ab92508.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C1QB or complement component 1 q subcomponent beta chain is a protein that plays a mechanical role in the complement system. It has a mass of approximately 25 kDa and is expressed in many tissues including the liver and blood plasma. C1QB is a subunit of the C1q complex which is the first component of the classical complement pathway. This protein works with C1q's other subunits to recognize and bind to the Fc region of antibodies facilitating the activation of the complement pathway.

Biological function summary

C1QB is involved in the immune response and helps in the clearance of pathogens and apoptotic cells. It forms part of the C1q complex which includes two other components: C1QA and C1QC. This complex binds to antibodies that are attached to antigens on the surface of pathogens activating the C1r and C1s serine proteases. The activated enzymes then cleave other complement proteins triggering a cascade that results in opsonization inflammation and cell lysis.

Pathways

C1QB takes a significant role in the classical complement pathway and humoral immunity. The classical pathway starts with the binding of the C1 complex to antigen-antibody complexes. Through this mechanism C1QB associates with proteins such as C2 and C4 which further propagate the complement activation cascade. The classical complement pathway links to the lectin pathway where mannose-binding lectin substitutes for C1q to recognize pathogens.

Defects or deficiencies in C1QB relate to systemic lupus erythematosus (SLE) and hereditary angioedema. In SLE impaired function of C1QB leads to decreased clearance of immune complexes contributing to autoimmune reactions. This protein interacts with C1QA in disease mechanisms. In hereditary angioedema although C1QB is not directly defective its involvement in complement activation relates to mutations in C1 inhibitor protein. These mutations result in uncontrolled complement activation underlying the pathophysiology of the disorder.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

See full target information C1QB

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C1QB antibody [EPR2981] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

Western blot - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] - BSA and Azide free (AB247575)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise