Anti-C1r antibody [EPR28027-35]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1r antibody [EPR28027-35] (AB324100)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling C1r with ab324100 at 1/2000 (0.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in human pancreas.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1r antibody [EPR28027-35] (AB324100)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling C1r with ab324100 at 1/2000 (0.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in human cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1r antibody [EPR28027-35] (AB324100)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling C1r with ab324100 at 1/2000 (0.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in plasma of human colon.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1r antibody [EPR28027-35] (AB324100)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C1r with ab324100 at 1/2000 (0.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human liver.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1r antibody [EPR28027-35] (AB324100)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling C1r with ab324100 at 1/2000 (0.27 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in plasma of human lung.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-C1r antibody [EPR28027-35] (AB324100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : pancreas

Full-length C1R is approximately 80 kDa. Band around 55kDa and 40 kDa might be cleavage fragment(PMID : 31879527).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 10 seconds, lane 2-4 : 59 seconds

All lanes:

Western blot - Anti-C1r antibody [EPR28027-35] (ab324100) at 1/1000 dilution

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Lane 3:

Human colon tissue lysate at 20 µg

Lane 4:

Human pancreas tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-C1r antibody [EPR28027-35] (AB324100)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Full-length C1R is approximately 80 kDa. Band around 55kDa and 40 kDa might be cleavage fragment(PMID : 31879527).

The expression of C1R is upregulated in response to BFA treatment (PMID : 8227070).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-C1r antibody [EPR28027-35] (ab324100) at 1/1000 dilution

Lane 1:

Untreated U-118 MG (human brain glioblastoma cell) whole cell lysate at 20 µg

Lane 2:

U-118 MG treated with 300ng/ml BFA for 24 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa,36 kDa

false

Exposure time: 109s