Skip to main content

Rabbit Recombinant Monoclonal C1S antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (AB248746), expandable thumbnail
  • Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (AB248746), expandable thumbnail
  • Western blot - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (AB248746), expandable thumbnail
  • Western blot - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (AB248746), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

For antigen retrieval: heat up to 98 degree C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. Cleaves also IGFBP5 and thereby inhibits the trophic effects of IGF1.

Alternative names

Complement C1s subcomponent, C1 esterase, Complement component 1 subcomponent s, C1S

Recommended products

Rabbit Recombinant Monoclonal C1S antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR9066(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248746 is the carrier-free version of Anti-C1s antibody [EPR9066(B)] ab134943.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (ab248746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (ab248746)

    This data was developed using Anti-C1s antibody [EPR9066(B)] ab134943, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling C1s using Anti-C1s antibody [EPR9066(B)] ab134943 at a 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (ab248746), expandable thumbnail

    Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (ab248746)

    This data was developed using Anti-C1s antibody [EPR9066(B)] ab134943, the same antibody clone in a different buffer formulation.Western blot analysis on Immunoprecipitation pellet from human plasma lysate using anti-C1s RabMAb (Anti-C1s antibody [EPR9066(B)] ab134943).

    All lanes: Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] (Anti-C1s antibody [EPR9066(B)] ab134943)

    Predicted band size: 63 kDa, 77 kDa

    Observed band size: 65 kDa

  • Western blot - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (ab248746), expandable thumbnail

    Western blot - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (ab248746)

    This data was developed using Anti-C1s antibody [EPR9066(B)] ab134943, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-C1s antibody [EPR9066(B)] (Anti-C1s antibody [EPR9066(B)] ab134943) at 1/1000 dilution

    Lane 1: Human fetal kidney lysate at 10 µg

    Lane 2: A431 cell lysate at 10 µg

    Lane 3: Human platelet lysate at 10 µg

    Lane 4: Human plasma lysate at 10 µg

    Lane 5: Human serum lysate at 10 µg

    Secondary

    All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 77 kDa

  • Western blot - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (ab248746), expandable thumbnail

    Western blot - Anti-C1s antibody [EPR9066(B)] - BSA and Azide free (ab248746)

    C1s Western blot staining using rabbit Anti-C1s antibody

    This data was developed using Anti-C1s antibody [EPR9066(B)] ab134943, the same antibody clone in a different buffer formulation.

    Western blot: Rabbit Monoclonal[EPR9066(B)] to C1s Anti-C1s antibody [EPR9066(B)] ab134943 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 77 kDa in Wild-type A549 cell lysates with no signal observed at this size in C1S knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-C1s antibody [EPR9066(B)] (Anti-C1s antibody [EPR9066(B)] ab134943) at 1/1000 dilution

    Lane 1: Wild-type A549 at 20 µg

    Lane 2: C1S knockout A549 at 20 µg

    Lane 3: HEK-293 at 20 µg

    Lane 4: Human Liver at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 77 kDa

    Observed band size: 77 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com