Anti-C1s antibody [EPR9067(B)] - BSA and Azide free

• WB

Unknown

Western blot - Anti-C1s antibody [EPR9067(B)] - BSA and Azide free (AB248737)

This data was developed using ab134928, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C1s antibody [EPR9067(B)] (<a href='/en-us/products/primary-antibodies/c1s-antibody-epr9067b-ab134928'>ab134928</a>) at 1/1000 dilution

Lane 1:

Human platelet lysate at 10 µg

Lane 2:

Human serum lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 77 kDa

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• WB

Lab

Western blot - Anti-C1s antibody [EPR9067(B)] - BSA and Azide free (AB248737)

This data was developed using ab134928, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal [EPR9067(B)] to C1s ab134928 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 80 kDa in Wild-type A549 cell lysates with no signal observed at this size in C1S knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-C1s antibody [EPR9067(B)] (<a href='/en-us/products/primary-antibodies/c1s-antibody-epr9067b-ab134928'>ab134928</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

C1S knockout A549 at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

HEK-293 at 20 µg

Lane 5:

Human Liver at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 77 kDa,80 kDa

Observed band size: 80 kDa,40 kDa

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• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-C1s antibody [EPR9067(B)] - BSA and Azide free (AB248737)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.