Anti-C2 antibody [EPR17979] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-C2 antibody [EPR17979] - BSA and Azide free (AB251512)

This data was developed using ab209900, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1 and 3 : 3 minutes; Lane 2 : 30 seconds.

All lanes:

Western blot - Anti-C2 antibody [EPR17979] (<a href='/en-us/products/primary-antibodies/c2-antibody-epr17979-ab209900'>ab209900</a>) at 1/1000 dilution

Lane 1:

Human serum at 10 µg

Lane 2:

Human plasma at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

Lanes 1 - 2:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Lane 3:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 83 kDa

Observed band size: 100 kDa

false

• WB

Supplier Data

Western blot - Anti-C2 antibody [EPR17979] - BSA and Azide free (AB251512)

This data was developed using ab209900, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-C2 antibody [EPR17979] (<a href='/en-us/products/primary-antibodies/c2-antibody-epr17979-ab209900'>ab209900</a>) at 1/1000 dilution

All lanes:

Mouse serum at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 83 kDa

Observed band size: 100 kDa

false

Exposure time: 1min