Rabbit Recombinant Monoclonal C21orf33 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (AB181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--immunoprecipitation-img107951.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (AB181366)")
![Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (AB181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--immunocytochemistry-immunofluorescence-img708.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (AB181366)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (AB181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129831.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (AB181366)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (AB181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95963.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (AB181366)")
![Western blot - Anti-C21orf33 antibody [EPR13213] (AB181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--western-blot-img99754.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C21orf33 antibody [EPR13213] (AB181366)")
Publications
Filter by
- BMC biology 20:682022GATD3A, a mitochondrial deglycase with evolutionary origins from gammaproteobacteria, restricts the formation of advanced glycation end products.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew J Smith,Jayshree Advani,Daniel C Brock,Jacob Nellissery,Jessica Gumerson,Lijin Dong,L Aravind,Breandán Kennedy,Anand SwaroopPubMed 35307029
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Predicted | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat mediated antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Alternative names
C21orf33, GATD3A, GATD3
Recommended products
Rabbit Recombinant Monoclonal C21orf33 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13213
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (ab181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--immunoprecipitation-img107951.jpg "Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (ab181366)")
Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (ab181366)
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or 1XPBS (lane 2) labeling C21orf33 using ab181366 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (ab181366)
Predicted band size: 25 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (ab181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--immunocytochemistry-immunofluorescence-img708.jpg "Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (ab181366)")
Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (ab181366)
Immunofluorescent staining of HepG2 cells labeling C21orf33 using ab181366 at a 1/100 dilution (red) with DAPI nuclear staining (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129831.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C21orf33 using ab181366 at a 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95963.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (ab181366)
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C21orf33 using ab181366 at a 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-C21orf33 antibody [EPR13213] (ab181366), expandable thumbnail](https://content.abcam.com/products/images/c21orf33-antibody-epr13213-ab181366--western-blot-img99754.jpg "Western blot - Anti-C21orf33 antibody [EPR13213] (ab181366)")
Western blot - Anti-C21orf33 antibody [EPR13213] (ab181366)
All lanes: Western blot - Anti-C21orf33 antibody [EPR13213] (ab181366) at 1/5000 dilution
Lane 1: A549 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
Predicted band size: 25 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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