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AB181366

Anti-C21orf33 antibody [EPR13213]

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(2 Publications)

Rabbit Recombinant Monoclonal C21orf33 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

C21orf33, GATD3A, GATD3

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (AB181366)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C21orf33 antibody [EPR13213] (AB181366)

Immunofluorescent staining of HepG2 cells labeling C21orf33 using ab181366 at a 1/100 dilution (red) with DAPI nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (AB181366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (AB181366)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C21orf33 using ab181366 at a 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (AB181366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] (AB181366)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C21orf33 using ab181366 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (AB181366)
  • IP

Supplier Data

Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (AB181366)

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or 1XPBS (lane 2) labeling C21orf33 using ab181366 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] (ab181366)

Predicted band size: 25 kDa

false

Western blot - Anti-C21orf33 antibody [EPR13213] (AB181366)
  • WB

Supplier Data

Western blot - Anti-C21orf33 antibody [EPR13213] (AB181366)

All lanes:

Western blot - Anti-C21orf33 antibody [EPR13213] (ab181366) at 1/5000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 25 kDa

false

  • Carrier free

    Anti-C21orf33 antibody [EPR13213] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13213

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GATD3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular psychiatry 30:3599-3614 PubMed40089604

2025

Shank3 modulates Rpl3 expression and protein synthesis via mGlu5: implications for Phelan McDermid syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Giona,Stefania Beretta,Antonio Zippo,Alessia Stefanoni,Zaira Tomasoni,Cinzia Vicidomini,Luisa Ponzoni,Mariaelvina Sala,Carrie K Jones,P Jeffrey Conn,Tobias M Boeckers,Carlo Sala,Chiara Verpelli

BMC biology 20:68 PubMed35307029

2022

GATD3A, a mitochondrial deglycase with evolutionary origins from gammaproteobacteria, restricts the formation of advanced glycation end products.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J Smith,Jayshree Advani,Daniel C Brock,Jacob Nellissery,Jessica Gumerson,Lijin Dong,L Aravind,Breandán Kennedy,Anand Swaroop
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com