AB224077

Anti-C2orf40/ECRG4 antibody

Name: Anti-C2orf40/ECRG4 antibody (ab224077) | Abcam
Brand: Abcam Limited
SKU: AB224077

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(5 Publications)

Rabbit Polyclonal C2orf40/ECRG4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ECRG4 aa 1 to C-terminus.

View Alternative Names

C2orf40, UNQ761/PRO1508, ECRG4, Augurin, Esophageal cancer-related gene 4 protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2orf40/ECRG4 antibody (AB224077)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2orf40/ECRG4 antibody (AB224077)

Paraffin embedded human skeletal muscle tissue stained for C2orf40/ECRG4 with ab224077 (1/200 dilution) in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2orf40/ECRG4 antibody (AB224077)

Paraffin embedded human placenta tissue stained for C2orf40/ECRG4 with ab224077 (1/200 dilution) in immunohistochemical analysis.

Supplier Data

Western blot - Anti-C2orf40/ECRG4 antibody (AB224077)

All lanes:

Western blot - Anti-C2orf40/ECRG4 antibody (ab224077) at 1/100 dilution

Lane 1:

Negative control (vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) ) cell lysate

Lane 2:

C2orf40/ECRG4 overexpression (with C-terminal myc-DDK tag, ~3.1 kDa) in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), cell lysate

Predicted band size: 17 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ECRG4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H1Z8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C2orf40 also known as ECRG4 (esophageal cancer related gene 4) is a protein with a molecular mass of approximately 14 kDa. It is expressed widely in human tissues including the brain testis and liver. ECRG4 functions as a membrane protein and plays a role in various cellular processes. Researchers often refer to it when studying its expression in normal and cancerous tissues due to its involvement in cell growth and regulation.

Biological function summary

ECRG4 participates in multiple cellular functions and takes part in neurogenesis and inflammation processes. The protein is not known to be part of a specific multiprotein complex. Its role extends to modulating cell cycle arrest and apoptosis indicating it has a regulatory function within the cell. Studies show its expression might influence tumor suppression and regeneration in tissue injury.

Pathways

ECRG4 plays a role in both the Wnt and NF-kB signaling pathways. These pathways are essential for cell proliferation and inflammation respectively. In the Wnt pathway ECRG4 can interact with proteins like beta-catenin to influence cell fate decisions. In the NF-kB signaling pathway this protein might modulate the transcription of genes that are part of immune responses and survival mechanisms.

ECRG4 has been linked to esophageal cancer and Alzheimer's disease. In cancer ECRG4 expression levels can impact tumor growth and might correlate with disease prognosis. When related to Alzheimer's this protein has connections with amyloid precursor protein which plays a significant role in the disease's pathology. ECRG4's varied roles in these disorders make it a focus of interest for therapeutic research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable hormone that may attenuate cell proliferation and induce senescence of oligodendrocyte and neural precursor cells in the central nervous system (By similarity). ECRG4-induced senescence is characterized by G1 arrest, RB1 dephosphorylation and accelerated CCND1 and CCND3 proteasomal degradation (By similarity).

See full target information ECRG4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 33:20 PubMed40958016

2025

CENP-A is diluted during bovine spermatogenesis and is maintained at internally positioned centromere clusters in mature bull sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Miriama Štiavnická,Anna Ní Nualláin,Caitríona M Collins,Elaine M Dunleavy

iScience 28:112608 PubMed40502710

2025

The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Wang,Haodong Li,Dong Fu,Yiming Zheng,Chuang Qian,Lin Li,Maoxiang Qian,Dahui Wang

Journal of biosciences 47: PubMed36226349

2022

UBR5 promotes migration and invasion of glioma cells by regulating the ECRG4/NF-ҡB pathway.

Applications

IHC, WB

Species

Mouse, Human

Qiang Wu,Ling Liu,Yan Feng,Liqun Wang,Xin Liu,Yanan Li

Journal of translational medicine 20:264 PubMed35676661

2022

C2orf40 inhibits metastasis and regulates chemo-resistance and radio-resistance of nasopharyngeal carcinoma cells by influencing cell cycle and activating the PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zuozhong Xie,Wei Li,Jingang Ai,Jun Xie,Xiaowei Zhang

Journal of tissue engineering and regenerative medicine 15:1162-1171 PubMed34551201

2021

Brain endothelial cells-derived extracellular vesicles overexpressing ECRG4 inhibit glioma proliferation through suppressing inflammation and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Huo,Song Yang,Haotian Wu,Yuchen Sun,Ranran Zhao,Ruihao Ye,Dongdong Yan,Xuefang Shi,Jiankai Yang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-C2orf40/ECRG4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2orf40/ECRG4 antibody (AB224077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2orf40/ECRG4 antibody (AB224077)

Western blot - Anti-C2orf40/ECRG4 antibody (AB224077)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise