Rabbit Polyclonal C3 antibody. Suitable for IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 31 publications.
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- Journal of neuroinflammation 20:2612023NeuroD1 administration ameliorated neuroinflammation and boosted neurogenesis in a mouse model of subarachnoid hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing Chen,Xue-Yan Liu,Mou-Hui Lin,Yu-Xi Li,De-Zhi Kang,Zu-Cheng Ye,Qing-Song LinPubMed 37953259
- Folia neuropathologica 61:273-2902023Astragaloside IV inhibits experimental autoimmune encephalomyelitis by modulating the polarization of both microglia/macrophages and astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingwen Yu,Bingtao Mu,Minfang Guo,Chunyun Liu,Tao Meng,Yuqing Yan,Lijuan Song,Jiezhong Yu,Gajendra Kumar,Cungen MaPubMed 37818688
- Cells 12:2023Differential Effects of Regulatory T Cells in the Meninges and Spinal Cord of Male and Female Mice with Neuropathic Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathan T Fiore,Brooke A Keating,Yuting Chen,Sarah I Williams,Gila Moalem-TaylorPubMed 37759539
- Journal of neuroinflammation 20:1132023β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuzhou Hua,Hong Zhu,Wen Yu,Qingcui Zheng,Lieliang Zhang,Weidong Liang,Yue Lin,Fan Xiao,Pengcheng Yi,Yanhong Xiong,Yao Dong,Hua Li,Lanran Fang,Hailin Liu,Jun Ying,Xifeng WangPubMed 37170230
- Frontiers in immunology 13:10213702022The extrafollicular response is sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLasse F Voss,Amanda J Howarth,Thomas R Wittenborn,Sandra Hummelgaard,Kristian Juul-Madsen,Kristian S Kastberg,Mathias K Pedersen,Lisbeth Jensen,Anastasios D Papanastasiou,Thomas Vorup-Jensen,Kathrin Weyer,Søren E DegnPubMed 36591222
- Journal of neuroinflammation 19:232022Fluoxetine inhibited the activation of A1 reactive astrocyte in a mouse model of major depressive disorder through astrocytic 5-HTR/β-arrestin2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinquan Fang,Xiao Ding,Yihe Zhang,Lei Cai,Yuan Ge,Kaiyang Ma,Rong Xu,Shanshan Li,Mengmeng Song,Hong Zhu,Jiaqi Liu,Jianhua Ding,Ming Lu,Gang HuPubMed 35093099
- Frontiers in pharmacology 12:7278742021Sanqi Oral Solution Mitigates Proteinuria in Rat Passive Heymann Nephritis and Blocks Podocyte Apoptosis Nrf2/HO-1 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowan Wang,Jinchu Liu,Ruimin Tian,Bidan Zheng,Chuang Li,Lihua Huang,Zhisheng Lu,Jing Zhang,Wei Mao,Bo Liu,Kun Bao,Peng XuPubMed 34867334
- Journal of cellular and molecular medicine 25:10291-103052021Targeting of EIF4EBP1 by miR-99a-3p affects the functions of B lymphocytes via autophagy and aggravates SLE disease progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Yang,Binbin Yang,Danqi DengPubMed 34668631
- Antioxidants (Basel, Switzerland) 10:2021Signal Transducer and Activator of Transcription 3 (STAT3) Suppresses STAT1/Interferon Signaling Pathway and Inflammation in Senescent Preadipocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAisha Y Madani,Yasser Majeed,Houari B Abdesselem,Maha V Agha,Muneera Vakayil,Nour K Al Sukhun,Najeeb M Halabi,Pankaj Kumar,Shahina Hayat,Mohamed A Elrayess,Arash Rafii,Karsten Suhre,Nayef A MazloumPubMed 33672392
- Frontiers in pharmacology 11:6189922021Induced Expression of kir6.2 in A1 Astrocytes Propagates Inflammatory Neurodegeneration via Drp1-dependent Mitochondrial Fission.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNanshan Song,Hong Zhu,Rong Xu,Jiaqi Liu,Yinquan Fang,Jing Zhang,Jianhua Ding,Gang Hu,Ming LuPubMed 33584303
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | IHC-Fr | |
|---|---|---|
| Mouse | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10 | Notes - |
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Target data
Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. The short isoform has B-cell stimulatory activity. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2.
Alternative names
Complement C3, HSE-MSF, C3
Recommended products
Rabbit Polyclonal C3 antibody. Suitable for IHC-P, IHC-Fr and reacts with Mouse samples. Cited in 31 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Protein G
- Specificity
This polyclonal antibody detects a band approximately 120 kDa in Western blot under reducing conditions, corresponding to the C3 alpha chain.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.
- Biological function summary
Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.
- Pathways
The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.
- Associated diseases and disorders
Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Image from Rutar M et al., Journal of Neuroinflammation 2012, 9:257, 26 November 2012. Fig 5.; doi: 10.1186/1742-2094-9-257.Immunohistochemistry (Frozen sections) - Anti-C3 antibody (ab11887)
Immunohistochemical analysis of rat retina tissue after bright continuous white light exposure, staining C3 with ab11887.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody (ab11887)
Positive staining of paraffin embedded mouse kidney tissue with ab11887 at 100x dilution.
Immunohistochemistry (Frozen sections) - Anti-C3 antibody (ab11887)
Positive staining of frozen mouse spleen tissue with ab11887 at 20x dilution.
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