Anti-C3 antibody (ab97462) is a rabbit polyclonal antibody detecting C3/C3b in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.
- Over 20 publications
- Trusted since 2010
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- iScience 26:1067012023Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Han H Hsu,Greta Pintacuda,Ruize Liu,Eugeniu Nacu,April Kim,Kalliopi Tsafou,Natalie Petrossian,William Crotty,Jung Min Suh,Jackson Riseman,Jacqueline M Martin,Julia C Biagini,Daya Mena,Joshua K T Ching,Edyta Malolepsza,Taibo Li,Tarjinder Singh,Tian Ge,Shawn B Egri,Benjamin Tanenbaum,Caroline R Stanclift,Annie M Apffel,Steven A Carr,Monica Schenone,Jake Jaffe,Nadine Fornelos,Hailiang Huang,Kevin C Eggan,Kasper LagePubMed 37207277
- Cell genomics 3:1002502023Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGreta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper LagePubMed 36950384
- Open medicine (Warsaw, Poland) 17:2117-21292022miRNA-130a-3p targets sphingosine-1-phosphate receptor 1 to activate the microglial and astrocytes and to promote neural injury under the high glucose condition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuang Yang,Jinxin ShiPubMed 36582210
- Frontiers in immunology 13:10359302022Complement component 3 protects human bronchial epithelial cells from cigarette smoke-induced oxidative stress and prevents incessant apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuqiang Pei,Jing Zhang,Jingge Qu,Yafei Rao,Danyang Li,Xiaoyan Gai,Yahong Chen,Ying Liang,Yongchang SunPubMed 36605203
- Oxidative medicine and cellular longevity 2022:38000042022Bu Shen Yi Sui Capsule Promotes Myelin Repair by Modulating the Transformation of A1/A2 Reactive Astrocytes and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Zha,Yi-Jiang Liu,Si-Si Liu,Nan Zhang,Jun-Ling Li,Fang Qi,Liang-Yun Jin,Bing Xue,Tao Yang,Yong-Ping Fan,Hui Zhao,Lei WangPubMed 36092158
- Journal of neuroinflammation 19:932022The smoothened agonist SAG reduces mitochondrial dysfunction and neurotoxicity of frataxin-deficient astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrés Vicente-Acosta,Alfredo Giménez-Cassina,Javier Díaz-Nido,Frida LoriaPubMed 35413853
- Frontiers in medicine 9:8456792022Activation of Complement Pathways in Kidney Tissue May Mediate Tubulointerstitial Injury in Diabetic Nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShimin Jiang,Yuanyuan Jiao,Guming Zou,Hongmei Gao,Li Zhuo,Wenge LiPubMed 35479942
- Journal of diabetes investigation 13:839-8492022Activation of complement C1q and C3 in glomeruli might accelerate the progression of diabetic nephropathy: Evidence from transcriptomic data and renal histopathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Jiao,Shimin Jiang,Ying Wang,Tianyu Yu,Guming Zou,Li Zhuo,Wenge LiPubMed 34932275
- Nature communications 12:62022021Lung mesenchymal stromal cells influenced by Th2 cytokines mobilize neutrophils and facilitate metastasis by producing complement C3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyuan Zheng,Ya-Nan Li,Shanfen Jia,Mengting Zhu,Lijuan Cao,Min Tao,Jingting Jiang,Shenghua Zhan,Yongjing Chen,Ping-Jin Gao,Weiguo Hu,Ying Wang,Changshun Shao,Yufang ShiPubMed 34707103
- Frontiers in cell and developmental biology 9:6916482021Neuron-Glia Crosstalk Plays a Major Role in the Neurotoxic Effects of Ketamine Extracellular Vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonald H Penning,Simona Cazacu,Aharon Brodie,Vesna Jevtovic-Todorovic,Steve N Kalkanis,Michael Lewis,Chaya BrodiePubMed 34604212
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human C3 aa 1450-1650. The exact immunogen used to generate this antibody is proprietary information. Database link P01024
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750, PubMed:2909530, PubMed:8376604, PubMed:9059512).
Alternative names
CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
Recommended products
Anti-C3 antibody (ab97462) is a rabbit polyclonal antibody detecting C3/C3b in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.
- Over 20 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human C3 aa 1450-1650. The exact immunogen used to generate this antibody is proprietary information. Database link P01024
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-C3 antibody (ab97462) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of C3/C3b?
Anti-C3 (ab97462) specifically detects a band for C3/C3b (UniProt: P01024) at a molecular weight of 187kDa.
Trusted by the scientific community
Anti-C3 (ab97462) was first used in a scientific publication in 2010 and has been cited over 20 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.
- Biological function summary
Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.
- Pathways
The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.
- Associated diseases and disorders
Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-C3 antibody (ab97462)
5% SDS-PAGE.
All lanes: Western blot - Anti-C3 antibody (ab97462) at 1/1000 dilution
Lane 1: Huh7 whole cell lysate at 30 µg
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 187 kDa
Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody (ab97462)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling C3 with ab97462 at 1/200 dilution (green) in ICC/IF. Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes.
Nuclei were stained using Hoechst 33342 (blue).
Immunoprecipitation - Anti-C3 antibody (ab97462)
C3 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts using 5 μg of ab97462. Western blot was performed from the immunoprecipitate using ab97462.
Lane 1: HepG2 whole cell extract.
Lane 2: Control IgG instead of ab97462 in HepG2 whole cell extract.
Lane 3: ab97462 IP in HepG2 whole cell extract.
All lanes: Immunoprecipitation - Anti-C3 antibody (ab97462)
Predicted band size: 187 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody (ab97462)
Paraffin-embedded mouse brain tissue stained for C3 using ab97462 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-C3 antibody (ab97462)
7.5% SDS-PAGE.
All lanes: Western blot - Anti-C3 antibody (ab97462) at 1/1500 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 187 kDa
Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody (ab97462)
ab97462 at 1/100 dilution staining C3 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody (ab97462)
ab97462 at 1/100 dilution staining C3 in H1299 xenograft by Immunohistochemistry, Paraffin-embedded tissue.
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