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Rabbit Recombinant Monoclonal C3 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.


Images

Western blot - Anti-C3 antibody [EPR2988] (AB181147), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [EPR2988] (AB181147), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-C3 antibody [EPR2988] (AB181147), expandable thumbnail
  • Western blot - Anti-C3 antibody [EPR2988] (AB181147), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

For unpurified use at 1/250 - 1/500.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/1000 - 1/10000.

Tested
Tested

Species
Human
Dilution info
1/10
Notes

For unpurified use at 1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750, PubMed:2909530, PubMed:8376604, PubMed:9059512).

Alternative names

CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

Recommended products

Rabbit Recombinant Monoclonal C3 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2988
Purification technique
Affinity purification Protein A
Specificity

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.

Biological function summary

Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.

Pathways

The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.

Associated diseases and disorders

Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-C3 antibody [EPR2988] (ab181147), expandable thumbnail

    Western blot - Anti-C3 antibody [EPR2988] (ab181147)

    The molecular weights observed are consistent with what have been described in the literature (PMID: 23619360)

    All lanes: Western blot - Anti-C3 antibody [EPR2988] (ab181147) at 1/1000 dilution

    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Human plasma lysate at 20 µg

    Lane 3: Human serum lysate at 20 µg

    Lane 4: Human spinal cord lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 187 kDa

    Observed band size: 115 kDa, 34 kDa, 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [EPR2988] (ab181147), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [EPR2988] (ab181147)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling C3 with purified ab181147 at 1/250 dilution (0.4 μg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-C3 antibody [EPR2988] (ab181147), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-C3 antibody [EPR2988] (ab181147)

    Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling C3 with purified ab181147 at 1/20 dilution (5 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-C3 antibody [EPR2988] (ab181147), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-C3 antibody [EPR2988] (ab181147)

    C3 western blot using anti-C3 antibody [EPR2988] ab181147. Publication image and figure legend from Schäfer, N., Rasras, A., et al., 2021, Front Immunol, PubMed 34819935.


    ab181147 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab181147 please see the product overview.

    C3aR expression was time-dependently regulated in FHR-3 stressed ARPE-19 cells. (A)C3AR mRNA expression was decreased after 5 h and increased after 24 h FHR-3 incubation. This effect could be confirmed on protein level: (B) Western blots of ARPE-19 cell lysates showed a tendency for decreased C3aR levels (54 kDa) 5 h after FHR-3 treatment. Supplementary Figure 1M shows full Western blots. (C) Decreased C3aR protein levels were detected by immunofluorescence using a specific anti-C3aR antibody (red) after 5 h FHR-3 incubation (upper panels), whereas no differences between FHR-3 stressed and unstressed controls were observed after 24 h (lower panels). Scale bars 40 µm. w/o untreated control (dotted line). Mean with standard deviation is shown. *p < 0.05. (A) Wilcoxon matched-pairs signed rank test (n = 3); (B) Ordinary one-way ANOVA, Turkey’s multiple comparisons test (n = 3).

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