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Mouse Monoclonal C3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (AB36385), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (AB36385), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (AB36385), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (AB36385), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/150

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Product

Target data

Function

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.C3-beta-cActs as a chemoattractant for neutrophils in chronic inflammation.Acylation stimulating proteinAdipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:8376604, PubMed:2909530, PubMed:9059512, PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750).

Alternative names

Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, C3

Recommended products

Mouse Monoclonal C3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications.

Alternative names

Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, C3

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

K13/16

Purification technique

Affinity purification Protein A

Specificity

Does not cross react with C4a or C5a.

Epitope

Recognizes an epitope present on human C3, C3a and C3a(desArg).

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Effectively inhibits the biological activity of C3a in a guinea-pig platelet activation assay.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

C3a also known as C3a des Arg or C3a full form is a small peptide anaphylatoxin derived from the complement component C3. It plays an important mechanical role in the immune system by acting as a potent mediator of inflammation. C3a weighs approximately 9 kDa. It expresses primarily on the surface of immune cells such as mast cells and basophils. The function of C3a involves the binding to the C3a receptor (C3aR) triggering a series of immune responses.

Biological function summary

C3a serves as an essential inflammatory agent and is part of the complement system an important component of the innate immune response. It contributes to the defense against pathogens by promoting chemotaxis degranulation and the release of histamine. C3a desArg a derivative of C3a generated by the removal of the C-terminal arginine residue has reduced inflammatory activity. The activity of C3a and its link to inflammation highlights its importance in maintaining immune homeostasis and responding to infectious threats.

Pathways

C3a plays an important role in the complement activation cascade particularly the classical and alternative pathways. This involvement highlights its relationship with proteins such as C3b and C5 which are critical in the opsonization and membrane attack complex formation. C3a's role in these pathways accentuates its importance in modulating inflammation and immune response.

Associated diseases and disorders

Abnormalities in C3a regulation have notable implications. C3a is associated with various inflammatory and autoimmune diseases like asthma and rheumatoid arthritis. In asthma C3a interacts with other complement proteins such as C5a to exacerbate inflammation in the airways. Dysregulation of C3a and its conversion to C3a desArg may further contribute to pathologies implicating its potential as a therapeutic target in managing inflammatory diseases.

Product promise

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (ab36385), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (ab36385)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling C3a / C3a des Arg with ab36385 at a dilution of 1/150. Strong staining is visible in the of veins of nephritis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (ab36385), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (ab36385)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C3a / C3a des Arg with ab36385 at a dilution of 1/150. Strong staining is visible in the veins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (ab36385), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (ab36385)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling C3a / C3a des Arg with ab36385 at a dilution of 1/150. Strong staining is visible in the lumen of veins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (ab36385), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a / C3a des Arg antibody [K13/16] (ab36385)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling C3a / C3a des Arg with ab36385 at a dilution of 1/75. Strong staining is visible in the of veins of nephritis.

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Product protocols

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