AB288413

Anti-C3a R antibody [3G7]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal C3a R antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human C3AR1.

View Alternative Names

AZ3B, C3R1, HNFAG09, C3AR1, C3a anaphylatoxin chemotactic receptor, C3AR, C3a-R

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-C3a R antibody [3G7] (AB288413)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C3a R antibody [3G7] (AB288413)

Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with ab288413 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with ab288413 at 10 μg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom ab288413 , DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

3G7

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human C3AR1. The exact immunogen used to generate this antibody is proprietary information.

Q16581

Specificity

Binds specifically to the 2e loop of the human C3a receptor, residues 185-193.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C3a receptor also known as C3aR or C3aR1 is a critical component of the immune system. It functions by binding to the C3a peptide a product of the complement cascade. This binding triggers a variety of immune responses. C3aR is a surface receptor widely expressed on immune cells such as macrophages mast cells and neutrophils. The molecular weight of the C3a receptor is approximately 55 kDa. It can be found principally in tissues involved in immunity like the spleen lungs and liver.

Biological function summary

The C3a receptor plays an important role in the immune response by mediating inflammatory reactions. It is not part of a larger complex like some receptors functioning instead through protein-protein interactions. Upon activation by binding with the C3a peptide the receptor can promote the release of histamine cytokines and other inflammatory mediators. This action helps regulate the body's defense against pathogens and contributes to the elimination of foreign materials from the body.

Pathways

The C3a receptor is involved in the complement pathway and immune response pathways. It interacts with several proteins within these pathways including C3a protein itself and other complement components. By engaging in these pathways C3aR significantly influences cellular responses and activities. For example its activation can lead to the alteration of immune cell behavior enhancing phagocytosis and increasing cell migration to sites of infection or injury.

The C3a receptor has been implicated in asthma and rheumatoid arthritis. In asthma C3aR activation can lead to overactive immune responses causing increased bronchoconstriction and airway hyperresponsiveness. Its involvement in rheumatoid arthritis relates to its ability to modulate inflammation exacerbating joint damage and discomfort. The receptor's activity in these conditions can also connect it to other proteins such as specific inflammatory cytokines which contribute to the pathological processes seen in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

See full target information C3AR1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70272 PubMed39777689

2025

The C3/C3aR pathway exacerbates acetaminophen-induced mouse liver injury via upregulating podoplanin on the macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanli Xie,Jiang Jiang,Fei Yang,Jingjing Han,Zhenni Ma,Tao Wen,Xia Bai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C3a R antibody [3G7]

Immunocytochemistry/ Immunofluorescence - Anti-C3a R antibody [3G7] (AB288413)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise