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Rabbit Recombinant Monoclonal C3a R antibody. Suitable for IHC-P, mIHC and reacts with Transfected cell line - Human, Human samples.

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Images

Multiplex immunohistochemistry - Anti-C3a R antibody [EPR28765-1] (AB317321), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (AB317321), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (AB317321), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (AB317321), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (AB317321), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PmIHCWBIPICC/IFFlow Cyt
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell line - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell line - Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell line - Human, Rat
Dilution info
-
Notes

-

Target data

Function

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

Alternative names

AZ3B, C3R1, HNFAG09, C3AR1, C3a anaphylatoxin chemotactic receptor, C3AR, C3a-R

Recommended products

Rabbit Recombinant Monoclonal C3a R antibody. Suitable for IHC-P, mIHC and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28765-1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The C3a receptor also known as C3aR or C3aR1 is a critical component of the immune system. It functions by binding to the C3a peptide a product of the complement cascade. This binding triggers a variety of immune responses. C3aR is a surface receptor widely expressed on immune cells such as macrophages mast cells and neutrophils. The molecular weight of the C3a receptor is approximately 55 kDa. It can be found principally in tissues involved in immunity like the spleen lungs and liver.

Biological function summary

The C3a receptor plays an important role in the immune response by mediating inflammatory reactions. It is not part of a larger complex like some receptors functioning instead through protein-protein interactions. Upon activation by binding with the C3a peptide the receptor can promote the release of histamine cytokines and other inflammatory mediators. This action helps regulate the body's defense against pathogens and contributes to the elimination of foreign materials from the body.

Pathways

The C3a receptor is involved in the complement pathway and immune response pathways. It interacts with several proteins within these pathways including C3a protein itself and other complement components. By engaging in these pathways C3aR significantly influences cellular responses and activities. For example its activation can lead to the alteration of immune cell behavior enhancing phagocytosis and increasing cell migration to sites of infection or injury.

Associated diseases and disorders

The C3a receptor has been implicated in asthma and rheumatoid arthritis. In asthma C3aR activation can lead to overactive immune responses causing increased bronchoconstriction and airway hyperresponsiveness. Its involvement in rheumatoid arthritis relates to its ability to modulate inflammation exacerbating joint damage and discomfort. The receptor's activity in these conditions can also connect it to other proteins such as specific inflammatory cytokines which contribute to the pathological processes seen in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Multiplex immunohistochemistry - Anti-C3a R antibody [EPR28765-1] (ab317321), expandable thumbnail

    Multiplex immunohistochemistry - Anti-C3a R antibody [EPR28765-1] (ab317321)

    C3a R immunohistochemistry staining of human tonsil using rabbit anti-C3a R antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining C3a R with ab317321 at a 1:1000 (0.52 ug/ml) dilution, Anti-CD14 antibody [SP192] ab183322 anti-CD14 used at 1:100 (1.22 ug/ml) dilution and Anti-CD3 epsilon antibody [SP7] ab16669 anti-CD3 epsilon used at a 1:150 (0.06 ug/ml) dilution.

    Panel A: merged staining of anti-C3AR1 (green; Opal™520) anti-CD14 (red; Opal™570) anti-CD3 epsilon (magenta; Opal™690) on human tonsil.
    Panel B: anti-C3AR1 stained on immune cells.
    Panel C: anti-CD14 stained on macrophages.
    Panel D: anti-CD3 epsilon stained on T lymphocytes.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab317321, Anti-CD14 antibody [SP192] ab183322 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a C3AR1 expression vector containing a his tag. (B) HEK-293T transfected with empty vector containing a his tag. tissue labeling C3a R with ab317321 at 1/1000 (0.517 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) HEK-293T transfected with a C3AR1 expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag.

    The section was incubated with ab317321 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling C3a R with ab317321 at 1/1000 (0.517 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on hofbauer cells of human placenta (PMID 22901903).

    The section was incubated with ab317321 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C3a R with ab317321 at 1/1000 (0.517 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on kupffer cells of human liver (PMID: 24974766).

    The section was incubated with ab317321 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321)

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling C3a R with ab317321 at 1/1000 (0.517 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on microglia of human astrocytoma (PMID: PMID: 37174113).

    The section was incubated with ab317321 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody [EPR28765-1] (ab317321)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling C3a R with ab317321 at 1/1000 (0.517 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on microglia of human cerebrum (PMID: 28002390).

    The section was incubated with ab317321 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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