Anti-C3AR1 antibody [HL2831] - BSA and Azide free

• Flow Cyt

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Flow Cytometry - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (AB325839)

ab325839 detects C3a Receptor protein by flow cytometry analysis. Sample : non-fixed and non-permeabilized HL-60 or HeLa cells were stained with 5 ug/ml (red) or a Rabbit monoclonal IgG isotype control (grey) in 1% FBS/ 5mM EDTA/PBS at 4ºC for 1 hour.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (AB325839)

ab325839 detects C3a Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded HL-60 xenograft. C3a Receptor stained by ab325839 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (AB325839)

ab325839 detects C3a Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample : Paraffin-embedded human glioblastoma. C3a Receptor stained by ab325839 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (AB325839)

ab325839 detects C3a Receptor protein by immunohistochemical analysis. Sample : Paraffin-embedded human cerebellum. C3a Receptor stained by ab325839 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

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Western blot - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (AB325839)

Untreated (–) and treated (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody ab325839 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (ab325839) at 1/1000 dilution

Lane 1:

Untreated (–) unboiled HL-60 whole cell extracts at 30 µg

Lane 2:

PNGase F, 2 hr treated (+) unboiled HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (AB325839)

ab325839 detects C3a Receptor protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : C3a Receptor stained by ab325839 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (AB325839)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325839 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-C3AR1 antibody [HL2831] - BSA and Azide free (ab325839) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged C3AR1 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged C3AR1 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false