Mouse Monoclonal C3 antibody. Carrier free. Suitable for WB, ELISA and reacts with Human samples.
Images
![Western blot - Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (AB234629), expandable thumbnail](https://content.abcam.com/products/images/c3b-ic3b-antibody-7c12-bsa-and-azide-free-ab234629--western-blot-img140041.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (AB234629)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ELISA | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
1 product for Alternative Version
Target data
Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750, PubMed:2909530, PubMed:8376604, PubMed:9059512).
Alternative names
CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
Recommended products
Mouse Monoclonal C3 antibody. Carrier free. Suitable for WB, ELISA and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 7C12
- Purification technique
- Affinity purification
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze
Notes
ab234629 is the carrier-free version of Anti-C3b / iC3b antibody [7C12] ab231078.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C3b protein also known as complement component 3b plays a significant role in the immune system aiding in the clearance of pathogens. It is a large glycoprotein with a mass of approximately 180 kDa. C3b is expressed mainly in the liver and released into the bloodstream as part of the complement system. Upon activation from its precursor C3 it becomes involved in opsonization enhancing the phagocytosis of pathogens by marking them for destruction by immune cells.
- Biological function summary
C3b acts as a critical mediator in the immune system by forming complexes with other complement proteins. It is part of the alternative and classical pathways of the complement system. C3b can also convert itself into iC3b after cleavage by factor I and iC3b continues to facilitate phagocytosis but with reduced ability to promote further complement activation. This conversion ensures homeostasis and regulation preventing overactivation of the immune system.
- Pathways
C3b participates in both the classical and alternative pathways of the complement system. It associates with proteins such as factor B and factor D to form the C3bBb complex in the alternative pathway acting as a C3 convertase that amplifies response. In the classical pathway C3b enhances the activity of C5 convertase facilitating the formation of the membrane attack complex. These pathways are vital in maintaining immune surveillance and response to infections.
- Associated diseases and disorders
Dysfunction or dysregulation of C3b activity links to autoimmune diseases and infectious diseases. For example atypical hemolytic uremic syndrome (aHUS) involves uncontrolled complement activation due to mutations affecting proteins like C3b. In such conditions C3b and related proteins such as factor H become therapeutic targets to modulate the complement system's overactivation. Studying the role of C3b in these diseases provides insight into potential interventions and novel treatments to manage complement-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (ab234629), expandable thumbnail](https://content.abcam.com/products/images/c3b-ic3b-antibody-7c12-bsa-and-azide-free-ab234629--western-blot-img140041.jpg "Western blot - Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (ab234629)")
Western blot - Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (ab234629)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk before Anti-C3b / iC3b antibody [7C12] ab231078 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-C3b / iC3b antibody [7C12] ab231078).
All lanes: Western blot - Anti-C3b / iC3b antibody [7C12] (Anti-C3b / iC3b antibody [7C12] ab231078)
Lane 1: Human serum diluted 1/100 at 5 µL
Lane 2: Human plasma diluted 1/100 at 5 µL
Lane 3: Human liver whole tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 187 kDa
Observed band size: 42 kDa
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