JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB221816

Anti-C3Orf34 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal C3Orf34 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CEP19 aa 50 to C-terminus.

View Alternative Names

C3orf34, HSD5, CEP19, Centrosomal protein of 19 kDa, Cep19

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-C3Orf34 antibody (AB221816)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C3Orf34 antibody (AB221816)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for C3Orf34 (green) using ab221816 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CEP19 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96LK0

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C3Orf34 also known as Chromosome 3 Open Reading Frame 34 is a protein of interest with an approximate mass of 41 kDa. This protein is expressed in various tissues with higher expression levels observed in certain brain regions and immune cells. Mechanically C3Orf34 functions as a component of cellular metabolism where it participates in specific biochemical reactions. It has a role in the regulation of energy production and cellular homeostasis. By providing structural support or acting as a catalytic enzyme C3Orf34 contributes to maintaining cellular function and integrity.
Biological function summary

C3Orf34 engages in critical cellular processes influencing cell growth and survival. It becomes part of multiprotein complexes essential for metabolite processing and energy distribution. Interaction within these complexes helps stabilize protein structures and allows effective metabolic flux. Researchers have found that C3Orf34 impacts cellular responses to metabolic stress thereby affecting how cells adapt to changes in their environment. Through these activities it contributes not only to regular cellular functions but also to overarching physiological processes.

Pathways

C3Orf34 participates in the central metabolic and signal transduction pathways including the glycolysis and AMPK pathways. The glycolytic pathway involves C3Orf34 in the process of breaking down glucose for energy while the AMPK pathway relies on its role in energy sensing and homeostasis. In these pathways C3Orf34 interacts with key proteins such as AMPK itself and enzymes involved in metabolic regulation. These interactions highlight its place in controlling metabolic rates adapting to energy demands and ensuring cellular energy balance.

C3Orf34 has links to metabolic disorders such as type 2 diabetes and neurodegenerative diseases. Aberrations in C3Orf34 function can disrupt metabolic balance and glucose homeostasis contributing to diabetes progression. In neurodegenerative diseases the protein associates with neuronal cell death processes. For these conditions C3Orf34 interacts with proteins like insulin receptor substrates in diabetes and amyloid precursor protein in neurodegenerative diseases. Understanding its role in these disorders offers insights into potential therapeutic targets for treating associated pathologies.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Required for ciliation (PubMed : 28428259, PubMed : 28625565, PubMed : 28659385). Recruits the RABL2B GTPase to the ciliary base to initiate ciliation. After specifically capturing the activated GTP-bound RABL2B, the CEP19-RABL2B complex binds intraflagellar transport (IFT) complex B from the large pool pre-docked at the base of the cilium and thus triggers its entry into the cilia (PubMed : 28428259, PubMed : 28625565). Involved in the early steps in cilia formation by recruiting the ciliary vesicles (CVs) to the distal end of the mother centriole where they fuse to initiate cilium assembly. Involved in microtubule (MT) anchoring to the centrosomes (PubMed : 28659385).
See full target information CEP19

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2404243121 PubMed39331410

2024

Subcellular activation of β-adrenergic receptors using a spatially restricted antagonist.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Liccardo,Johannes Morstein,Ting-Yu Lin,Julius Pampel,Di Lang,Kevan M Shokat,Roshanak Irannejad
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com