AB11863

Anti-C4 antibody [16D2]

Name: Anti-C4 antibody [16D2] (ab11863) | Abcam
Brand: Abcam Limited
SKU: AB11863
Price: 545 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(17 Publications)

Rat Monoclonal C4 antibody. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 17 publications.

View Alternative Names

C4, C4b, Complement C4-B

1 Images

Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (AB11863)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (AB11863)

Frozen section of mouse pulmonary artery of control (left) and mouse with hypoxia-induced pulmonary hypertnetion (right). Staining of C4 (red) with ab11863 in a dilution 1/300. Green is autofluorescence of elastic lamellae defining vascular media. Blue is cell nucleus.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

16D2

Isotype

IgG2a

Carrier free

Reacts with

Mouse

Applications

IHC-Fr

applications

Specificity

The monoclonal antibody 16D2 reacts with intact mouse C4, C4b and C4d.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 4 (C4) is a significant protein in the complement system also known as C4 complement. C4 has a molecular mass of approximately 206 kDa and mainly expresses in the liver. It is present in the blood plasma. C4 plays an important role in the immunological response and activates classical and lectin complement pathways. It serves a mechanical function by binding to pathogen surfaces and facilitating the cleavage that leads to the downstream activation of the complement cascade. There's a C4 ELISA test that allows for quantifying C4 levels in serum which is important for clinical diagnostics.

Biological function summary

Complement C4 participates in the immune defense by marking pathogens for destruction and enhancing phagocytosis. C4 is a part of a complex with other complement proteins like C3 and C1 to initiate the formation of the C3 convertase enzyme. This action amplifies the immune response leading to the clearance of microorganisms and apoptotic cells. C4 also plays a role in regulating the inflammation process maintaining homeostasis in the immune system.

Pathways

C4 fits into the complement activation pathway specifically the classical and lectin pathways. These pathways are important for innate immunity and connect with components like complement C3 and complement C1. Through the classical pathway C4 gets activated after binding to antibodies that have recognized antigens leading to a chain reaction that strengthens the immune response. The lectin pathway also requires C4 activation through different stimuli solidifying C4's central role in immune system signaling networks.

Abnormal C4 activity associates with autoimmune diseases like systemic lupus erythematosus (SLE) and rheumatoid arthritis. Low levels of complement C4 in serum can indicate these autoimmune conditions where the immune system mistakenly attacks its own tissues. C4 also connects to other proteins like complement component C1q in the context of SLE. C4 deficiencies or abnormalities may contribute to the development or exacerbation of these diseases highlighting its importance in disease pathogenesis and highlighting its value as a target for diagnostic tests such as C4 ELISA or serum complement tests.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens (By similarity).

See full target information C4b

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40710297

2025

Complement Modulation Mitigates Inflammation-Mediated Preterm Birth and Fetal Neural Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Eliza R McElwee,Devin Hatchell,Mohammed Alshareef,Khalil Mallah,Harriet Hall,Hannah Robinson,Ramin Eskandari,Eugene Chang,Scott Sullivan,Stephen Tomlinson

iScience 28:111930 PubMed40034849

2025

Complement is primarily activated in the lung in a mouse model of severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Peter J Szachowicz,Christine Wohlford-Lenane,Cobey J Donelson,Shreya Ghimire,Andrew Thurman,Biyun Xue,Timothy J Boly,Abhishek Verma,Leila MašinoviĆ,Jennifer R Bermick,Tayyab Rehman,Stanley Perlman,David K Meyerholz,Alejandro A Pezzulo,Yuzhou Zhang,Richard J H Smith,Paul B McCray

International journal of molecular sciences 25: PubMed39000309

2024

Mannan-Binding Lectin Is Associated with Inflammation and Kidney Damage in a Mouse Model of Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Gry H Dørflinger,Charlotte B Holt,Steffen Thiel,Jesper N Bech,Jakob A Østergaard,Mette Bjerre

Nature communications 13:6233 PubMed36280666

2022

Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Zhou,Yuxin Li,Xiaoyu Li,Fanzhuo Zeng,Yanxia Rao,Yang He,Yafei Wang,Meizhen Liu,Dali Li,Zhen Xu,Xin Zhou,Siling Du,Fugui Niu,Jiyun Peng,Xifan Mei,Sheng-Jian Ji,Yousheng Shu,Wei Lu,Feifan Guo,Tianzhun Wu,Ti-Fei Yuan,Ying Mao,Bo Peng

International journal of molecular sciences 22: PubMed34502128

2021

Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Enzbrenner,Rahel Zulliger,Josef Biber,Ana Maria Quintela Pousa,Nicole Schäfer,Corinne Stucki,Nicolas Giroud,Marco Berrera,Elod Kortvely,Roland Schmucki,Laura Badi,Antje Grosche,Diana Pauly,Volker Enzmann

Infection and immunity 87: PubMed31182619

2019

Persistent Infection of the Brain Induced Neurodegeneration Associated with Activation of Complement and Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,Emily G Severance,Raphael P Viscidi,Robert H Yolken,Jianchun Xiao

Brain, behavior, and immunity 80:889-899 PubMed31158497

2019

Microglial cell hyper-ramification and neuronal dendritic spine loss in the hippocampus and medial prefrontal cortex in a mouse model of PTSD.

Applications

Unspecified application

Species

Unspecified reactive species

Kristie Leigh Smith,Mustafa S Kassem,David J Clarke,Michael P Kuligowski,Miguel A Bedoya-Pérez,Stephanie M Todd,Jim Lagopoulos,Maxwell R Bennett,Jonathon C Arnold

Frontiers in immunology 9:3038 PubMed30728823

2019

Germinal Center Alloantibody Responses Mediate Progression of Chronic Allograft Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Manu Chhabra,Jawaher Alsughayyir,M Saeed Qureshi,Mekhola Mallik,Jason M Ali,Ivonne Gamper,Ellen L Moseley,Sarah Peacock,Vasilis Kosmoliaptsis,Martin J Goddard,Michelle A Linterman,Reza Motallebzadeh,Gavin J Pettigrew

Cell reports 15:1214-27 PubMed27134179

2016

Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant.

Applications

Unspecified application

Species

Unspecified reactive species

Ines G Harper,Jason M Ali,Simon J F Harper,Elizabeth Wlodek,Jawaher Alsughayyir,Margaret C Negus,M Saeed Qureshi,Reza Motalleb-Zadeh,Kourosh Saeb-Parsy,Eleanor M Bolton,J Andrew Bradley,Menna R Clatworthy,Thomas M Conlon,Gavin J Pettigrew

American journal of transplantation : official jou 14:2263-2274 PubMed25155089

2014

Depletion of foxp3(+) T cells abrogates tolerance of skin and heart allografts in murine mixed chimeras without the loss of mixed chimerism.

Applications

Unspecified application

Species

Unspecified reactive species

K Shinoda,T Akiyoshi,C M Chase,E A Farkash,D K Ndishabandi,C M Raczek,D P Sebastian,P Della Pelle,P S Russell,J C Madsen,R B Colvin,A Alessandrini

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C4 antibody [16D2]

Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (AB11863)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

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