Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Plasma staining on Human bladder carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling C4 binding protein/C4BPB with ab199430 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows : -
-ve control 1 - ab199430 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

C4 binding protein/C4BPB was immunoprecipitated from 1mg of Human serum with ab199430 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab199430 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : Human serum 10 μg. Lane 2 : Human serum following immunoprecipitation. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199430 in Human serum.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] (<a href='/en-us/products/primary-antibodies/c4-binding-protein-c4bpb-antibody-epr17101-ab199430'>ab199430</a>)

Predicted band size: 28 kDa

Observed band size: 45 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 2300577).

All lanes:

Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (<a href='/en-us/products/primary-antibodies/c4-binding-protein-c4bpb-antibody-epr17101-ab199430'>ab199430</a>) at 1/10000 dilution

All lanes:

Human serum at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 45 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 2300577).

All lanes:

Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (<a href='/en-us/products/primary-antibodies/c4-binding-protein-c4bpb-antibody-epr17101-ab199430'>ab199430</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human ovary cancer extract. at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa

Observed band size: 45 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 2300577).

All lanes:

Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (<a href='/en-us/products/primary-antibodies/c4-binding-protein-c4bpb-antibody-epr17101-ab199430'>ab199430</a>) at 1/1000 dilution

All lanes:

Rat plasma at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa,37 kDa,73 kDa

Observed band size: 42 kDa,45 kDa,73 kDa

false

Exposure time: 30s