Rabbit Recombinant Monoclonal C4 binding protein/C4BPB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat samples.
pH: 7.2 - 7.4
Constituents: PBS
ICC/IF | IP | WB | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Rat | Expected | Expected | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
C4b-binding protein beta chain, C4BPB
Rabbit Recombinant Monoclonal C4 binding protein/C4BPB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat samples.
pH: 7.2 - 7.4
Constituents: PBS
ab251274 is the carrier-free version of Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
C4 binding protein also known as C4BPB is a vital component of the complement system responsible for immune function. It has a molecular mass of approximately 570 kDa and is expressed mainly in liver tissues. C4BPB is a multi-chain protein complex consisting of several alpha and beta chains. This structure allows it to perform important roles in the regulation of immune responses. The presence of C4BPB can be detected in the serum where it acts to modulate the activity of complement C4 protein.
C4 binding protein plays a defensive role in the immune system by regulating complement activation. It is part of a larger complex that controls the complement pathway which is integral to immune surveillance and removing pathogens. C4BPB functions primarily by binding to complement C4 preventing the formation of the C3 convertase which is an enzyme that amplifies the complement response. This binding action helps in stabilizing the protein C4 and complement C4 serum to avoid unnecessary immune reactions.
The complement system pathway prominently features C4 binding protein. In the classical complement activation pathway C4BPB works closely with other proteins such as C3 and C4 to ensure regulatory balance. Its inhibitory action on the complement C4 pathway provides a safeguard against damage to host tissues during immune responses. The interplay between C4BPB and protein C4 offers a protective mechanism reducing excessive inflammation which can lead to tissue injury.
C4 binding protein holds relevance in conditions like rheumatoid arthritis and systemic lupus erythematosus (SLE). Both diseases involve dysregulation of the immune system where inappropriate complement activation occurs. C4BPB's ability to inhibit complement C4 activation suggests that disruptions in its function may contribute to the pathophysiology of these autoimmune disorders. Additionally the relationship between C4BPB and other complement proteins like C3 and C4 is critical in understanding the underlying mechanisms driving these diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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This data was developed using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 2300577).
All lanes: Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430) at 1/1000 dilution
Lane 1: Human plasma at 20 µg
Lane 2: Human ovary cancer extract. at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 45 kDa
Exposure time: 1min
This data was developed using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 2300577).
All lanes: Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430) at 1/10000 dilution
All lanes: Human serum at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 45 kDa
Exposure time: 1min
This data was developed using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 2300577).
All lanes: Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430) at 1/1000 dilution
All lanes: Rat plasma at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa, 37 kDa, 73 kDa
Observed band size: 42 kDa, 45 kDa, 73 kDa
Exposure time: 30s
This data was developed using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling C4 binding protein/C4BPB with Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling C4 binding protein/C4BPB with Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Plasma staining on Human bladder carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430, the same antibody clone in a different buffer formulation.
C4 binding protein/C4BPB was immunoprecipitated from 1mg of Human serum with Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430 at 1/80 dilution. Western blot was performed from the immunoprecipitate using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human serum 10 μg. Lane 2: Human serum following immunoprecipitation. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430 in Human serum.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] (Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430)
Predicted band size: 28 kDa
Observed band size: 45 kDa
This data was developed using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C4 binding protein/C4BPB with Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling C4 binding protein/C4BPB with Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
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