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Rabbit Recombinant Monoclonal C4 binding protein/C4BPB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat samples.

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Images

Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274), expandable thumbnail
  • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274), expandable thumbnail
  • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (AB251274), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Tested
Tested
Tested
Tested
Rat
Expected
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal C4 binding protein/C4BPB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR17101
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251274 is the carrier-free version of Anti-C4 binding protein/C4BPB antibody [EPR17101] ab199430.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

C4 binding protein also known as C4BPB is a vital component of the complement system responsible for immune function. It has a molecular mass of approximately 570 kDa and is expressed mainly in liver tissues. C4BPB is a multi-chain protein complex consisting of several alpha and beta chains. This structure allows it to perform important roles in the regulation of immune responses. The presence of C4BPB can be detected in the serum where it acts to modulate the activity of complement C4 protein.

Biological function summary

C4 binding protein plays a defensive role in the immune system by regulating complement activation. It is part of a larger complex that controls the complement pathway which is integral to immune surveillance and removing pathogens. C4BPB functions primarily by binding to complement C4 preventing the formation of the C3 convertase which is an enzyme that amplifies the complement response. This binding action helps in stabilizing the protein C4 and complement C4 serum to avoid unnecessary immune reactions.

Pathways

The complement system pathway prominently features C4 binding protein. In the classical complement activation pathway C4BPB works closely with other proteins such as C3 and C4 to ensure regulatory balance. Its inhibitory action on the complement C4 pathway provides a safeguard against damage to host tissues during immune responses. The interplay between C4BPB and protein C4 offers a protective mechanism reducing excessive inflammation which can lead to tissue injury.

Associated diseases and disorders

C4 binding protein holds relevance in conditions like rheumatoid arthritis and systemic lupus erythematosus (SLE). Both diseases involve dysregulation of the immune system where inappropriate complement activation occurs. C4BPB's ability to inhibit complement C4 activation suggests that disruptions in its function may contribute to the pathophysiology of these autoimmune disorders. Additionally the relationship between C4BPB and other complement proteins like C3 and C4 is critical in understanding the underlying mechanisms driving these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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