Rabbit Recombinant Monoclonal C4 binding protein/C4BPB antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (AB322888), expandable thumbnail](https://content.abcam.com/products/images/c4-binding-protein-c4bpb-antibody-epr26617-563-bsa-and-azide-free-capture-ab322888--sandwich-elisa-img440299.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (AB322888)")
![Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (AB322888), expandable thumbnail](https://content.abcam.com/products/images/c4-binding-protein-c4bpb-antibody-epr26617-563-bsa-and-azide-free-capture-ab322888--sandwich-elisa-img440300.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (AB322888)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
Alternative names
C4b-binding protein beta chain, C4BPB
Rabbit Recombinant Monoclonal C4 binding protein/C4BPB antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26617-563
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab322888 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human C4 binding protein/C4BPB. The recommended pair for sandwich ELISA is:
Capture: ab322888, Human C4 binding protein/C4BPB Capture Antibody (unconjugated)
Detector: Anti-C4 binding protein/C4BPB antibody [EPR26617-579] - BSA and Azide free (Detector) ab322889, Human C4 binding protein/C4BPB Detector Antibody (unconjugated)
The reference range value is 15.6-2000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (ab322888), expandable thumbnail](https://content.abcam.com/products/images/c4-binding-protein-c4bpb-antibody-epr26617-563-bsa-and-azide-free-capture-ab322888--sandwich-elisa-img440299.jpg "Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (ab322888)")
Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (ab322888)
Sandwich ELISA of Human C4 binding protein/C4BPB Antibody Pair - BSA and Azide free ab322887 with the capture antibody ab322888 dilution at 2 ug/mL and detector antibody Anti-C4 binding protein/C4BPB antibody [EPR26617-579] - BSA and Azide free (Detector) ab322889 dilution at 0.5 ug/mL.
![Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (ab322888), expandable thumbnail](https://content.abcam.com/products/images/c4-binding-protein-c4bpb-antibody-epr26617-563-bsa-and-azide-free-capture-ab322888--sandwich-elisa-img440300.jpg "Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (ab322888)")
Sandwich ELISA - Anti-C4 binding protein/C4BPB antibody [EPR26617-563] - BSA and Azide free (Capture) (ab322888)
Interpolated concentration of native C4 binding protein/C4BPB was measured at different sample concentrations. Undiluted samples are as follows: human serum 10% and human plasma (EDTA) 10%. Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
Downloads
Product protocols
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