AB87429

Anti-C4a antibody

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(1 Publication)

Rabbit Polyclonal C4A antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CO4, CPAMD2, C4A, Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2

1 Images

Unknown

Western blot - Anti-C4a antibody (AB87429)

Complement C4-A circulates in blood as a disulphide linked trimer of an alpha, beta and gamma chain (95, 75 and 33 kDa respectively). Our immunogen sequence is within the region corresponding to the alpha chain (95 kDa).

All lanes:

Western blot - Anti-C4a antibody (ab87429) at 2 µg/mL

All lanes:

Human Plasma Total Protein Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 193 kDa

Observed band size: 95 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement C4a also known as C4a anaphylatoxin is a component of the complement system with a molecular mass of approximately 9 kDa. This small protein originates from the proteolytic cleavage of complement component C4 which contributes to immune system activation. C4a is mainly expressed in the liver but can also be produced by other tissues during inflammation. It serves as a chemoattractant recruiting immune cells to sites of infection helping initiate a response against pathogens.

Biological function summary

Complement C4a acts as an anaphylatoxin participating in the complex immune system response. Anaphylatoxins like C4a C3a and C5a contribute to the inflammatory process by inducing smooth muscle contraction increasing vascular permeability and triggering histamine release from mast cells and basophils. C4a complements the activities of other complement components playing an important role in modulating inflammation and defense mechanisms in the body.

Pathways

The activation of C4a is an important step within the classical and lectin pathways of complement activation. These pathways involve the recognition and elimination of pathogens and unwanted cells. C4a along with its related proteins C4b and components C2 and C3 facilitates the formation of C3 convertase which amplifies the response and further clears pathogens. C4a also interacts with complement inhibitors which regulate these pathways to prevent excessive tissue damage during inflammation.

C4a anaphylatoxin has associations with autoimmune diseases and angioneoplasm. Changes in serum levels of C4a are observed in conditions like systemic lupus erythematosus where it may contribute to tissue damage. High C4a levels can correlate with disease activity and severity. Additionally C4a alongside other anaphylatoxins like C3a and C5a might play a role in angiogenesis influencing tumor progression and angiogenesis-related disorders serving as a potential biomarker for disease severity and prognosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

See full target information C4A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 10:M111.011023 PubMed21799047

2011

A serum protein profile predictive of the resistance to neoadjuvant chemotherapy in advanced breast cancers.

Applications

Species

Human

Seok-Won Hyung,Min Young Lee,Jong-Han Yu,Byunghee Shin,Hee-Jung Jung,Jong-Moon Park,Wonshik Han,Kyung-Min Lee,Hyeong-Gon Moon,Hui Zhang,Ruedi Aebersold,Daehee Hwang,Sang-Won Lee,Myeong-Hee Yu,Dong-Young Noh

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