AB168358

Anti-C4B-d / C4B-b antibody [EPR11203]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal C4B-d / C4B-b antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CO4, CPAMD3, C4B_2, C4B, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

3 Images

Flow Cytometry (Intracellular) - Anti-C4B-d / C4B-b antibody [EPR11203] (AB168358)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-C4B-d / C4B-b antibody [EPR11203] (AB168358)

Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling C4b with ab168358 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

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Western blot - Anti-C4B-d / C4B-b antibody [EPR11203] (AB168358)

All lanes:

Western blot - Anti-C4B-d / C4B-b antibody [EPR11203] (ab168358) at 1/1000 dilution

Lane 1:

Human serum lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 193 kDa

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Lab

Western blot - Anti-C4B-d / C4B-b antibody [EPR11203] (AB168358)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 5:

Western blot - Anti-C4B-d / C4B-b antibody [EPR11203] (ab168358) at 1/1000 dilution

Lanes 6 - 10:

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (<a href='/en-us/products/primary-antibodies/6x-his-tag-antibody-epr20547-chip-grade-ab213204'>ab213204</a>) at 1/5000 dilution

Lanes 1 and 6:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lanes 2 and 7:

293T cells transfected with a human C4d-A (Uniprot: P0C0L4 aa957-1336) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 3 and 8:

293T cells transfected with a human C4d-B (Uniprot: P0C0L5 aa957-1336) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 4 and 9:

293T cells transfected with a human C4-A alpha chain (Uniprot: P0C0L4 aa680- 1446) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 5 and 10:

293T cells transfected with a human C4b-A (Uniprot: P0C0L4 aa757-1446) expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45-100 kDa

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Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11203

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'C4b' protein also known as the 'C4b complement' plays a significant role in the immune system. This protein is a part of the larger complement system specifically as a segment derived from the cleavage of the native 'C4 complement'. It has an estimated molecular mass of about 200 kDa. The 'C4b' protein is prominently expressed on the surface of cells and in body fluids where the immune response is active. As an important player in the complement pathway it binds covalently to foreign particles labeling them for destruction or removal by immune cells.

Biological function summary

The 'C4b complement' interacts with various other components of the immune system. It is particularly involved in the formation of the C3 and C5 convertase complexes which are essential for the opsonization and activation cascade leading to pathogen elimination. Its attachment to cellular surfaces provides a docking point for proteins such as C2 facilitating the further cleavage and progression of the complement cascade. This interaction is critical for driving the classical and lectin pathways of complement activation.

Pathways

The 'C4b protein' is integral to the classical pathway of complement activation as well as to the lectin pathway. It associates with proteins like C1s C1q and factor B forming complexes that execute various stages of immune defenses. By forming C3 and C5 convertase 'C4b' not only promotes phagocytosis but also inflammation and cell lysis through membrane attack complexes. These processes underlie the body's ability to address microbial invasions efficiently.

'C4b complement dysregulation' can lead to autoimmune conditions such as systemic lupus erythematosus (SLE) and type 1 diabetes. In SLE reduced or malfunctional 'C4b' increases susceptibility to infections and triggers inappropriate immune responses. It can also correlate with the deficiency of related proteins like C2 which further impairs the complement system. These associations indicate that proper regulation of 'C4b' is essential for maintaining immune system stability and preventing overactive immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

See full target information C4B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:15743 PubMed31673107

2019

Structure Based Multitargeted Molecular Docking Analysis of Selected Furanocoumarins against Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Reetuparna Acharya,Shinu Chacko,Pritha Bose,Antonio Lapenna,Shakti Prasad Pattanayak

Clinical proteomics 13:26 PubMed27708557

2016

Plasma degradome affected by variable storage of human blood.

Applications

Species

Human

Maria Kaisar,Leon F A van Dullemen,Marie-Laëtitia Thézénas,M Zeeshan Akhtar,Honglei Huang,Sandrine Rendel,Philip D Charles,Roman Fischer,Rutger J Ploeg,Benedikt M Kessler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C4B-d / C4B-b antibody [EPR11203]

Flow Cytometry (Intracellular) - Anti-C4B-d / C4B-b antibody [EPR11203] (AB168358)

Western blot - Anti-C4B-d / C4B-b antibody [EPR11203] (AB168358)

Western blot - Anti-C4B-d / C4B-b antibody [EPR11203] (AB168358)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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