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Rabbit Recombinant Monoclonal C4BPA antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (AB251309), expandable thumbnail
  • Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (AB251309), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (AB251309), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (AB251309), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.

Alternative names

C4BP, C4BPA, C4b-binding protein alpha chain, C4bp, Proline-rich protein, PRP

Recommended products

Rabbit Recombinant Monoclonal C4BPA antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14780
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251309 is the carrier-free version of Anti-C4BPA antibody [EPR14780] ab200345.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

C4BPA also known as C4b-binding protein alpha chain is an important component in the immune system. It has a molecular mass of approximately 570 kDa. This protein is mainly expressed in the liver and is secreted into the plasma. C4BPA functions by binding to complement component 4b which is a part of the complement system responsible for managing immune responses and clearing pathogens.

Biological function summary

C4BPA plays a significant role in the regulation of the complement cascade specifically the classical and lectin pathways. It acts as a cofactor for factor I-mediated cleavage of C4b thereby inhibiting the progression of the complement activation. C4BPA forms a complex with other proteins such as C4BPB and this complex is essential for regulating the complement system and preventing damage to host cells.

Pathways

C4BPA is deeply involved in both the classical and lectin complement pathways. These pathways are important for antigen clearance and inflammation. C4BPA works together with proteins like complement component 3 (C3) to efficiently regulate these pathways preventing excessive complement activation. It associates with downstream effectors maintaining balance in immune defense mechanisms and inflammation control.

Associated diseases and disorders

C4BPA shows significant connections to autoimmune diseases and infections. Deficiencies or malfunctions in C4BPA can lead to conditions such as systemic lupus erythematosus where its regulatory role on complement activation becomes compromised. In infections C4BPA interacts with proteins like factor H playing a role in modulating host responses to prevent tissue damage. Understanding the interactions and regulatory processes involving C4BPA is important for developing therapeutic strategies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309), expandable thumbnail

    Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)

    This data was developed using Anti-C4BPA antibody [EPR14780] ab200345, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-C4BPA antibody [EPR14780] (Anti-C4BPA antibody [EPR14780] ab200345) at 1/5000 dilution

    All lanes: Human plasma lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 11 kDa, 41 kDa, 57 kDa, 58 kDa, 67 kDa

    Observed band size: 11 kDa, 57 kDa, 58 kDa, 67 kDa

    Exposure time: 30s

  • Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309), expandable thumbnail

    Western blot - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)

    This data was developed using Anti-C4BPA antibody [EPR14780] ab200345, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-C4BPA antibody [EPR14780] (Anti-C4BPA antibody [EPR14780] ab200345) at 1/5000 dilution

    Lane 1: Human fetal liver tissue lysate at 20 µg

    Lane 2: Human fetal kidney tissue lysate at 20 µg

    Lane 3: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Lane 4: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa

    Observed band size: 67 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)

    This data was developed using Anti-C4BPA antibody [EPR14780] ab200345, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling C4BPA using Anti-C4BPA antibody [EPR14780] ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of Anti-C4BPA antibody [EPR14780] ab200345 and secondary antibody only.
    Note: Plasma staining on human transitional cell carcinoma of bladder tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] - BSA and Azide free (ab251309)

    This data was developed using Anti-C4BPA antibody [EPR14780] ab200345, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling C4BPA using Anti-C4BPA antibody [EPR14780] ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of Anti-C4BPA antibody [EPR14780] ab200345 and secondary antibody only.
    Note: No staining on human skeletal muscle tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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