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AB182140

Anti-C4BPA antibody [EPR14781(B)] - C-terminal

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(2 Publications)

Rabbit Recombinant Monoclonal C4BPA antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

C4BP, C4BPA, C4b-binding protein alpha chain, C4bp, Proline-rich protein, PRP

3 Images
Immunoprecipitation - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (AB182140)
  • IP

Supplier Data

Immunoprecipitation - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (AB182140)

Western blot analysis of C4BPA in Human plasma immunoprecipitated with ab182140 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140)

Predicted band size: 67 kDa

false

Western blot - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (AB182140)
  • WB

Supplier Data

Western blot - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (AB182140)

All lanes:

Western blot - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/2000 dilution

All lanes:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 67 kDa

Observed band size: 70 kDa

false

Western blot - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (AB182140)
  • WB

Supplier Data

Western blot - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (AB182140)

All lanes:

Western blot - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/10000 dilution

All lanes:

Human plasma lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 67 kDa

Observed band size: 70 kDa

false

  • Carrier free

    Anti-C4BPA antibody [EPR14781(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14781(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C4BPA also known as C4b-binding protein alpha chain is an important component in the immune system. It has a molecular mass of approximately 570 kDa. This protein is mainly expressed in the liver and is secreted into the plasma. C4BPA functions by binding to complement component 4b which is a part of the complement system responsible for managing immune responses and clearing pathogens.
Biological function summary

C4BPA plays a significant role in the regulation of the complement cascade specifically the classical and lectin pathways. It acts as a cofactor for factor I-mediated cleavage of C4b thereby inhibiting the progression of the complement activation. C4BPA forms a complex with other proteins such as C4BPB and this complex is essential for regulating the complement system and preventing damage to host cells.

Pathways

C4BPA is deeply involved in both the classical and lectin complement pathways. These pathways are important for antigen clearance and inflammation. C4BPA works together with proteins like complement component 3 (C3) to efficiently regulate these pathways preventing excessive complement activation. It associates with downstream effectors maintaining balance in immune defense mechanisms and inflammation control.

C4BPA shows significant connections to autoimmune diseases and infections. Deficiencies or malfunctions in C4BPA can lead to conditions such as systemic lupus erythematosus where its regulatory role on complement activation becomes compromised. In infections C4BPA interacts with proteins like factor H playing a role in modulating host responses to prevent tissue damage. Understanding the interactions and regulatory processes involving C4BPA is important for developing therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It also interacts with anticoagulant protein S and with serum amyloid P component.
See full target information C4BPA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18022 PubMed37929660

2023

PIN1P1 is activated by CREB1 and promotes gastric cancer progression via interacting with YBX1 and upregulating PIN1.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Wen Wang,Wen-Jie Zhu,Ran-Ran Ma,Ya-Ru Tian,Xu Chen,Peng Gao

Malaria journal 19:155 PubMed32295584

2020

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Tougan,Jyotheeswara R Edula,Masayuki Morita,Eizo Takashima,Hajime Honma,Takafumi Tsuboi,Toshihiro Horii
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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