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AB136921

Anti-C4d antibody [A24-T]

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(4 Publications)

Rabbit Monoclonal C4b antibody. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human C4B.

View Alternative Names

CO4, CPAMD3, C4B_2, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [A24-T] (AB136921)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [A24-T] (AB136921)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human transplanted kidney tissue labelling C4d with ab136921 at 1/100 dilution showing diffuse strong positive immunostaining of dilated peritubular moderate capillaries indicating acute antibody mediated rejection.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [A24-T] (AB136921)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [A24-T] (AB136921)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human transplanted kidney tissue labelling C4d with ab136921 at 1/100 dilution showing diffuse strong positive immunostaining of peritubular and glomerular capillaries, indicating acute antibody mediated rejection.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

A24-T

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human C4B. The exact immunogen used to generate this antibody is proprietary information.

P0C0L5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
Storage buffer
pH: 8 Preservative: 0.05% Sodium azide Constituents: 2% BSA, 0.32% Tris HCl
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C4d also known as the C4d complement is a degradation product of the complement component C4. It has a molecular weight of approximately 45 kDa. This protein is commonly expressed on the surfaces of endothelial cells in various tissues where it plays a role in the immune response. The presence of C4d on tissues is often detected using techniques like immunohistochemistry (IHC) also referred to as C4d IHC or C4d immunostain providing valuable insights into complement activation.
Biological function summary

The C4d protein acts as a marker for complement activation and is part of the larger complement cascade. C4d is primarily associated with the classical pathway and serves as an indicator of complement-mediated cell injury. The degradation of C4 into C4d is usually seen in immune responses where antibodies recognize antigens triggering sequence activation in the complement pathway. C4d's role as a marker facilitates the differentiation between antibody-mediated injury and other types of tissue damage.

Pathways

This complement protein gets involved in the classical pathway of the complement system which plays a critical role in innate and adaptive immunity. It is related to other proteins like C3 and C1q which are additional components within this pathway. These interactions are significant for the clearance of pathogens and also assist in enhancing the humoral immune response by promoting the deposition of complement on pathogenic surfaces.

C4d presence becomes particularly significant in conditions like transplant rejection and systemic lupus erythematosus (SLE). In transplant rejection its deposition on tissue samples might indicate antibody-mediated rejection where C4d binds to the tissue signaling an immune response against the transplanted organ. Additionally in SLE C4d's relation to other proteins such as C3 and C1q confirms its involvement in autoimmune responses where the immune system mistakenly targets the body's own tissues leading to chronic inflammation and damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
See full target information C4B
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101744 PubMed39317190

2024

Pig-to-human kidney xenotransplants using genetically modified minipigs.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wang,Gang Chen,Dengke Pan,Hui Guo,Hongtao Jiang,Jianli Wang,Hao Feng,Songzhe He,Jiaxiang Du,Man Zhang,Tao Li,Yong Wang,Hang Yu,Huiling Gan,Quan Wen,Zhian Song,Desheng Li,Yifan Yu,Huanliang Wang,Bing Li,Yong You,Shen Zhou,Mingfa Wang,Lili Liu,Liang Xu,Meng Yang,Hua Pei,Kang Zhang,Zhonghua K Chen

Nature communications 15:2795 PubMed38555382

2024

Enzymatic conversion of human blood group A kidneys to universal blood group O.

Applications

Unspecified application

Species

Unspecified reactive species

Serena MacMillan,Sarah A Hosgood,Léonie Walker-Panse,Peter Rahfeld,Spence S Macdonald,Jayachandran N Kizhakkedathu,Stephen G Withers,Michael L Nicholson

Chinese medical journal 134:2874-2881 PubMed34711716

2021

Donor-specific antibodies, glomerulitis, and human leukocyte antigen B eplet mismatch are risk factors for peritubular capillary C4d deposition in renal allografts.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zheng,Hui Guo,Hui-Lin Gong,Ping Lan,Chen-Guang Ding,Yang Li,Xiao-Ming Ding,Wu-Jun Xue

FASEB journal : official publication of the Federa 31:2785-2796 PubMed28314768

2017

infection liberates transmissible, cytotoxic prion amyloids.

Applications

Unspecified application

Species

Unspecified reactive species

Ron Balczon,K Adam Morrow,Chun Zhou,Bradley Edmonds,Mikhail Alexeyev,Jean-Francois Pittet,Brant M Wagener,Stephen A Moser,Silas Leavesley,Xiangming Zha,Dara W Frank,Troy Stevens
View all publications
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