Rabbit Polyclonal C4b antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
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- FASEB journal : official publication of the Federa 33:12723-127342019Combining MAP-1:CD35 or MAP-1:CD55 fusion proteins with pattern-recognition molecules as novel targeted modulators of the complement cascade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Pérez-Alós et. al.PubMed 31469600
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
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Target data
Function
Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Alternative names
CO4, CPAMD3, C4B_2, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
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Rabbit Polyclonal C4b antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C4d also known as the C4d complement is a degradation product of the complement component C4. It has a molecular weight of approximately 45 kDa. This protein is commonly expressed on the surfaces of endothelial cells in various tissues where it plays a role in the immune response. The presence of C4d on tissues is often detected using techniques like immunohistochemistry (IHC) also referred to as C4d IHC or C4d immunostain providing valuable insights into complement activation.
- Biological function summary
The C4d protein acts as a marker for complement activation and is part of the larger complement cascade. C4d is primarily associated with the classical pathway and serves as an indicator of complement-mediated cell injury. The degradation of C4 into C4d is usually seen in immune responses where antibodies recognize antigens triggering sequence activation in the complement pathway. C4d's role as a marker facilitates the differentiation between antibody-mediated injury and other types of tissue damage.
- Pathways
This complement protein gets involved in the classical pathway of the complement system which plays a critical role in innate and adaptive immunity. It is related to other proteins like C3 and C1q which are additional components within this pathway. These interactions are significant for the clearance of pathogens and also assist in enhancing the humoral immune response by promoting the deposition of complement on pathogenic surfaces.
- Associated diseases and disorders
C4d presence becomes particularly significant in conditions like transplant rejection and systemic lupus erythematosus (SLE). In transplant rejection its deposition on tissue samples might indicate antibody-mediated rejection where C4d binds to the tissue signaling an immune response against the transplanted organ. Additionally in SLE C4d's relation to other proteins such as C3 and C1q confirms its involvement in autoimmune responses where the immune system mistakenly targets the body's own tissues leading to chronic inflammation and damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody (ab87424)
IHC image of C4d staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87424, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-C4d antibody (ab87424)
C4d circulates in blood as a disulfide-linked trimer of an alpha, beta and gamma chain. Our immunogen sequence is within the alpha chain. The band at 85kDa could correspond to the alpha chain.
All lanes: Western blot - Anti-C4d antibody (ab87424) at 1 µg/mL
All lanes: Human Plasma Total Protein Lysate (ab83997) at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 193 kDa
Observed band size: 110 kDa, 130 kDa, 193 kDa, 30 kDa, 48 kDa, 85 kDa
Exposure time: 30s
Immunocytochemistry/ Immunofluorescence - Anti-C4d antibody (ab87424)
ICC image of ab87424 stained HepG2 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87424, 5μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa, and MCF-7 cells at 5μg/ml.
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