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AB249610

Anti-C4d antibody [EPR10143] - BSA and Azide free

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Rabbit Recombinant Monoclonal C4d antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CO4, CPAMD3, C4B_2, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)

This data was developed using ab170942, the same antibody clone in a different buffer formulation.

ab170942 showing +ve staining in Human normal lung tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)

This data was developed using ab170942, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling C4a using ab170942 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)

This data was developed using ab170942, the same antibody clone in a different buffer formulation.

ab170942 showing +ve staining in Human thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)

This data was developed using ab170942, the same antibody clone in a different buffer formulation.

ab170942 showing +ve staining in Human normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)

This data was developed using ab170942, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling C4a with ab170942 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)

This data was developed using ab170942, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling C4a with ab170942 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)
  • WB

Lab

Western blot - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)

This data was developed using ab170942, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 5:

Western blot - Anti-C4d antibody [EPR10143] (<a href='/en-us/products/primary-antibodies/c4d-antibody-epr10143-ab170942'>ab170942</a>) at 1/1000 dilution

Lanes 6 - 10:

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (<a href='/en-us/products/primary-antibodies/6x-his-tag-antibody-epr20547-chip-grade-ab213204'>ab213204</a>) at 1/5000 dilution

Lanes 1 and 6:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lanes 2 and 7:

293T cells transfected with a human C4d-A (Uniprot: P0C0L4 aa957-1336) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 3 and 8:

293T cells transfected with a human C4d-B (Uniprot: P0C0L5 aa957-1336) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 4 and 9:

293T cells transfected with a human C4-A alpha chain (Uniprot: P0C0L4 aa680- 1446) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 5 and 10:

293T cells transfected with a human C4b-A (Uniprot: P0C0L4 aa757-1446) expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45-100 kDa

false

Exposure time: 1s

Western blot - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)
  • WB

Supplier Data

Western blot - Anti-C4d antibody [EPR10143] - BSA and Azide free (AB249610)

This data was developed using ab170942, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C4d antibody [EPR10143] (<a href='/en-us/products/primary-antibodies/c4d-antibody-epr10143-ab170942'>ab170942</a>) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Predicted band size: 193 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10143

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249610 is the carrier-free version of ab170942.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
See full target information C4B
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com