Rabbit Recombinant Monoclonal C4d antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] (AB170917), expandable thumbnail](https://content.abcam.com/products/images/c4d-antibody-epr11241-ab170917--flow-cytometry-intracellular-img94855.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] (AB170917)")
![Western blot - Anti-C4d antibody [EPR11241] (AB170917), expandable thumbnail](https://content.abcam.com/products/images/c4d-antibody-epr11241-ab170917--western-blot-img57751.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C4d antibody [EPR11241] (AB170917)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | IHC | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
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Target data
Function
Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Alternative names
CO4, CPAMD3, C4B_2, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
Recommended products
Rabbit Recombinant Monoclonal C4d antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11241
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] (ab170917), expandable thumbnail](https://content.abcam.com/products/images/c4d-antibody-epr11241-ab170917--flow-cytometry-intracellular-img94855.jpg "Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] (ab170917)")
Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] (ab170917)
C4d Flow Cytometry (Intracellular) staining using rabbit Anti-C4d antibody
Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling C4a with ab170917 at 1/100 (red) or a rabbit IgG negative (green).
![Western blot - Anti-C4d antibody [EPR11241] (ab170917), expandable thumbnail](https://content.abcam.com/products/images/c4d-antibody-epr11241-ab170917--western-blot-img57751.jpg "Western blot - Anti-C4d antibody [EPR11241] (ab170917)")
Western blot - Anti-C4d antibody [EPR11241] (ab170917)
C4d Western blot staining using rabbit Anti-C4d antibody
All lanes: Western blot - Anti-C4d antibody [EPR11241] (ab170917) at 1/1000 dilution
Lane 1: Human angioneoplasm lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
SecondaryAll lanes: Goat ant-rabbit HRP conjugated antibody at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 193 kDa
Downloads
Product protocols
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- Visit troubleshooting
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