Rabbit Recombinant Monoclonal C4d antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] - BSA and Azide free (AB249589), expandable thumbnail](https://content.abcam.com/products/images/c4d-antibody-epr11241-bsa-and-azide-free-ab249589--flow-cytometry-intracellular-img94855.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] - BSA and Azide free (AB249589)")
![Western blot - Anti-C4d antibody [EPR11241] - BSA and Azide free (AB249589), expandable thumbnail](https://content.abcam.com/products/images/c4d-antibody-epr11241-bsa-and-azide-free-ab249589--western-blot-img57751.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-C4d antibody [EPR11241] - BSA and Azide free (AB249589)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Alternative names
CO4, CPAMD3, C4B_2, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
Recommended products
Rabbit Recombinant Monoclonal C4d antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11241
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249589 is the carrier-free version of Anti-C4d antibody [EPR11241] ab170917.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] - BSA and Azide free (ab249589), expandable thumbnail](https://content.abcam.com/products/images/c4d-antibody-epr11241-bsa-and-azide-free-ab249589--flow-cytometry-intracellular-img94855.jpg "Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] - BSA and Azide free (ab249589)")
Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR11241] - BSA and Azide free (ab249589)
C4d Flow Cytometry (Intracellular) staining using rabbit Anti-C4d antibody
This data was developed using Anti-C4d antibody [EPR11241] ab170917, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling C4a with Anti-C4d antibody [EPR11241] ab170917 at 1/100 (red) or a rabbit IgG negative (green).
![Western blot - Anti-C4d antibody [EPR11241] - BSA and Azide free (ab249589), expandable thumbnail](https://content.abcam.com/products/images/c4d-antibody-epr11241-bsa-and-azide-free-ab249589--western-blot-img57751.jpg "Western blot - Anti-C4d antibody [EPR11241] - BSA and Azide free (ab249589)")
Western blot - Anti-C4d antibody [EPR11241] - BSA and Azide free (ab249589)
C4d Western blot staining using rabbit Anti-C4d antibody
This data was developed using Anti-C4d antibody [EPR11241] ab170917, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-C4d antibody [EPR11241] (Anti-C4d antibody [EPR11241] ab170917) at 1/1000 dilution
Lane 1: Human angioneoplasm lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
SecondaryAll lanes: Goat ant-rabbit HRP conjugated antibody at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 193 kDa
Downloads
Product protocols
- Visit the general protocols
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