Anti-C4d antibody [EPR2990(2)] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C4d antibody [EPR2990(2)] - BSA and Azide free (AB249774)

This data was developed using ab173577, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of HepG2 cells labeling C4 with ab173577 at 1/100

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-C4d antibody [EPR2990(2)] - BSA and Azide free (AB249774)

This data was developed using ab173577, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling C4 (red) with ab173577 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

• WB

Supplier Data

Western blot - Anti-C4d antibody [EPR2990(2)] - BSA and Azide free (AB249774)

This data was developed using ab173577, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C4d antibody [EPR2990(2)] (<a href='/en-us/products/primary-antibodies/c4d-antibody-epr29902-ab173577'>ab173577</a>) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Predicted band size: 193 kDa

false

• WB

Lab

Western blot - Anti-C4d antibody [EPR2990(2)] - BSA and Azide free (AB249774)

This data was developed using ab173577, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1 - 5:

Western blot - Anti-C4d antibody [EPR2990(2)] (<a href='/en-us/products/primary-antibodies/c4d-antibody-epr29902-ab173577'>ab173577</a>) at 1/1000 dilution

Lanes 6 - 10:

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (<a href='/en-us/products/primary-antibodies/6x-his-tag-antibody-epr20547-chip-grade-ab213204'>ab213204</a>) at 1/5000 dilution

Lanes 1 and 6:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lanes 2 and 7:

293T cells transfected with a human C4d-A (Uniprot: P0C0L4 aa957-1336) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 3 and 8:

293T cells transfected with a human C4d-B (Uniprot: P0C0L5 aa957-1336) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 4 and 9:

293T cells transfected with a human C4-A alpha chain (Uniprot: P0C0L4 aa680- 1446) expression vector containing a His-tag whole cell lysate at 20 µg

Lanes 5 and 10:

293T cells transfected with a human C4b-A (Uniprot: P0C0L4 aa757-1446) expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45-100 kDa

false

Exposure time: 1s