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AB167093

Anti-C4d antibody [LP69]

5

(2 Reviews)

|

(5 Publications)

Mouse Monoclonal C4b antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human C4B.

View Alternative Names

CO4, CPAMD3, C4B_2, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [LP69] (AB167093)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4d antibody [LP69] (AB167093)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney transplant rejection tissue labeling C4d with ab167093 at 1/25 dilution.

  • HRP

    HRP Anti-C4d antibody [LP69]

  • 519 FITC

    FITC Anti-C4d antibody [LP69]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LP69

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human C4B.

P0C0L5

Specificity

ab167093 reacts with an antigenic determinant in the C4d domain of C4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.065% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C4d also known as the C4d complement is a degradation product of the complement component C4. It has a molecular weight of approximately 45 kDa. This protein is commonly expressed on the surfaces of endothelial cells in various tissues where it plays a role in the immune response. The presence of C4d on tissues is often detected using techniques like immunohistochemistry (IHC) also referred to as C4d IHC or C4d immunostain providing valuable insights into complement activation.
Biological function summary

The C4d protein acts as a marker for complement activation and is part of the larger complement cascade. C4d is primarily associated with the classical pathway and serves as an indicator of complement-mediated cell injury. The degradation of C4 into C4d is usually seen in immune responses where antibodies recognize antigens triggering sequence activation in the complement pathway. C4d's role as a marker facilitates the differentiation between antibody-mediated injury and other types of tissue damage.

Pathways

This complement protein gets involved in the classical pathway of the complement system which plays a critical role in innate and adaptive immunity. It is related to other proteins like C3 and C1q which are additional components within this pathway. These interactions are significant for the clearance of pathogens and also assist in enhancing the humoral immune response by promoting the deposition of complement on pathogenic surfaces.

C4d presence becomes particularly significant in conditions like transplant rejection and systemic lupus erythematosus (SLE). In transplant rejection its deposition on tissue samples might indicate antibody-mediated rejection where C4d binds to the tissue signaling an immune response against the transplanted organ. Additionally in SLE C4d's relation to other proteins such as C3 and C1q confirms its involvement in autoimmune responses where the immune system mistakenly targets the body's own tissues leading to chronic inflammation and damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
See full target information C4B
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1506 PubMed39929829

2025

Enzymatic conversion of blood group B kidney prevents hyperacute antibody-mediated injuries in ABO-incompatible transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zeng,Ming Ma,Xiaojuan Jiang,Zhengsheng Rao,Dan Huang,Hao Zhang,Saifu Yin,Rong Bao,Haohan Zhang,Zhiling Wang,Hongwei Gao,Feng Gong,Tao Lin,Keqin Zhang,Turun Song

International journal of general medicine 16:1879-1889 PubMed37213477

2023

Lectin Complement Pathway Activation is Associated with Massive Proteinuria in PLA2R-Positive Membranous Nephropathy: A Retrospective Study.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Li,Jiao Zhang,Xu Wang,Xumin Zheng,Hongmei Gao,Shimin Jiang,Wenge Li

Frontiers in medicine 9:832998 PubMed35174193

2022

C4d as a Screening Tool and an Independent Predictor of Clinical Outcomes in Lupus Nephritis and IgA Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqian Yang,Yanhong Yuan,Xinghua Shao,Huihua Pang,Xiajing Che,Liou Cao,Minfang Zhang,Yao Xu,Zhaohui Ni,Chaojun Qi,Qin Wang,Shan Mou

Journal of clinical immunology 36:517-27 PubMed27146825

2016

Testing the Activity of Complement Convertases in Serum/Plasma for Diagnosis of C4NeF-Mediated C3 Glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Anna M Blom,Fernando Corvillo,Michal Magda,Grzegorz Stasiłojć,Pilar Nozal,Miguel Ángel Pérez-Valdivia,Virginia Cabello-Chaves,Santiago Rodríguez de Córdoba,Margarita López-Trascasa,Marcin Okrój

Circulation 127:1290-9 PubMed23443736

2013

Donor brain death exacerbates complement-dependent ischemia/reperfusion injury in transplanted hearts.

Applications

IHC

Species

Human

Carl Atkinson,Bernhard Floerchinger,Fei Qiao,Sarah Casey,Tucker Williamson,Ellen Moseley,Serban Stoica,Martin Goddard,Xupeng Ge,Stefan G Tullius,Stephen Tomlinson
View all publications
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