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AB243827

Anti-C4orf27 antibody

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(1 Publication)

Rabbit Polyclonal C4orf27 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HPF1 aa 50-200.

View Alternative Names

C4orf27, HPF1, Histone PARylation factor 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-C4orf27 antibody (AB243827)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C4orf27 antibody (AB243827)

Human cell line U-251 stained for C4orf27 using ab243827 at 2 μg/ml in Immunocytochemistry/immunofluorescence analysis.

Western blot - Anti-C4orf27 antibody (AB243827)
  • WB

Supplier Data

Western blot - Anti-C4orf27 antibody (AB243827)

All lanes:

Western blot - Anti-C4orf27 antibody (ab243827) at 0.4 µg/mL

All lanes:

MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HPF1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NWY4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C4orf27 also known as UPF0710 protein C4orf27 is a protein encoded by the C4orf27 gene. It has a molecular mass of approximately 14 kDa. The C4orf27 protein's mechanical function is not fully understood but it is believed to have a role in cellular processes due to its conserved sequences. Expression of C4orf27 is observed in various tissues including the liver kidney and brain suggesting its involvement in multiple physiological functions.
Biological function summary

The function of C4orf27 remains unspecified but appears to be involved in cellular homeostasis and metabolism. It is hypothesized to participate in intracellular signaling pathways although it does not form part of any well-studied protein complex. Researchers continue to investigate its specific biological roles and interactions within the cell due to its broad expression pattern.

Pathways

C4orf27 may interact with cellular signaling and metabolic pathways. While its direct involvement in specific pathways remains under study it shows potential interactions with pathways such as the mitochondrial function and oxidative phosphorylation. Proteins such as COX4I1 involved in the electron transport chain may relate to C4orf27 through these pathways suggesting a role in energy utilization and cellular stress responses.

Research is ongoing to understand C4orf27's contributions to pathological states. Initial studies suggest associations with metabolic disorders and neurodegenerative diseases such as Alzheimer's disease. It may interact with proteins like APP (Amyloid precursor protein) through oxidative stress pathways possibly linking it to the pathogenesis of neurodegeneration. Further research will clarify the relevance of C4orf27 in these diseases aiming to discover potential therapeutic implications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cofactor for serine ADP-ribosylation that confers serine specificity on PARP1 and PARP2 and plays a key role in DNA damage response (PubMed : 28190768, PubMed : 29480802, PubMed : 29954836, PubMed : 32028527, PubMed : 32939087, PubMed : 33186521, PubMed : 33589610, PubMed : 33683197, PubMed : 34108479, PubMed : 34210965, PubMed : 34486521, PubMed : 34625544, PubMed : 34732825, PubMed : 34795260, PubMed : 34874266). Initiates the repair of double-strand DNA breaks : recruited to DNA damage sites by PARP1 and PARP2 and switches the amino acid specificity of PARP1 and PARP2 from aspartate or glutamate to serine residues, licensing serine ADP-ribosylation of target proteins (PubMed : 28190768, PubMed : 29480802, PubMed : 29954836, PubMed : 32028527, PubMed : 32939087, PubMed : 33589610, PubMed : 33683197, PubMed : 34486521, PubMed : 34625544, PubMed : 34732825, PubMed : 34795260, PubMed : 34874266). Serine ADP-ribosylation of target proteins, such as histones, promotes decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed : 27067600, PubMed : 28190768, PubMed : 32939087, PubMed : 33589610). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed : 29480802). HPF1 acts by completing the active site of PARP1 and PARP2 : forms a composite active site composed of residues from HPF1 and PARP1 or PARP2 (PubMed : 32028527, PubMed : 33589610). While HPF1 promotes the initiation of serine ADP-ribosylation, it restricts the polymerase activity of PARP1 and PARP2 in order to limit the length of poly-ADP-ribose chains (PubMed : 33683197, PubMed : 34732825, PubMed : 34795260). HPF1 also promotes tyrosine ADP-ribosylation, probably by conferring tyrosine specificity on PARP1 (PubMed : 29954836, PubMed : 30257210).
See full target information HPF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular histology 56:144 PubMed40285932

2025

Identification of the oncogenic role of centromere protein M in non-small cell lung cancer via CDC20/MYBL2/Wnt signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wu,Jun Li,Haoyu Wang,Xu Chang,Qinglong Kong
View all publications
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Product promise

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