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AB185315

Anti-C4orf48 antibody

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(3 Publications)

Rabbit Polyclonal C4orf48 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NICOL1 aa 1-100.

View Alternative Names

C4orf48, NICOL1, NELL2-interacting cell ontogeny regulator 1, NELL2-interacting cofactor for lumicrine signaling, NICOL

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4orf48 antibody (AB185315)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4orf48 antibody (AB185315)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling C4orf48 in the cytoplasm of neuronal cells with ab185315 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-C4orf48 antibody (AB185315)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C4orf48 antibody (AB185315)

Immunocytochemistry analysis of PC-3 (human prostate adenocarcinoma cell line) whole cells labeling C4orf48 in the cytosol with ab185315 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4orf48 antibody (AB185315)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4orf48 antibody (AB185315)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling C4orf48 in the cytoplasm of neuronal cells with ab185315 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NICOL1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q5BLP8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C4orf48 is a protein encoded by the C4orf48 gene with an approximate mass of 16 kDa. Researchers also refer to it as Chromosome 4 open reading frame 48. The protein demonstrates expression across various tissues although detailed tissue-specific expression is not well characterized at present. Mechanically C4orf48 plays a role in cellular functions but the exact mechanisms remain under investigation. It does not belong to any broadly known protein families or structural groups.
Biological function summary

C4orf48 contributes to cellular processes such as growth and development. Current studies reveal no specific involvement in a larger protein complex suggesting it might function independently or as part of transient molecular interactions. Further investigations are necessary to establish detailed biological roles and interactions of C4orf48.

Pathways

Current data suggests that C4orf48 interacts with cellular pathways influencing transcription and signaling. It seems to have interactions with pathways potentially involving histological modulation though precise partners and downstream effects need clarification. Researchers have suggested potential crosstalk with proteins involved in regulatory networks but no definitive pathway integration has been identified yet.

C4orf48 may have links to specific conditions such as certain cancers or metabolic abnormalities. Ongoing studies work towards understanding its involvement in these diseases and whether it associates with known disease-related proteins. C4orf48's links to disorders need more robust exploration to concretize its significance in pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

mRNA-binding protein which interacts with a range of target mRNAs including SERPINE1, ACTA2, CCN2 and COL4A1 and may promote extracellular matrix production. Binds to the 3'-UTR of SERPINE1 mRNA and stabilizes the mRNA, possibly by competing for binding with SERBP1 and preventing SERBP1-mediated mRNA degradation. Also binds to the 3'-UTR of ACTA2. Testis-derived lumicrine factor that triggers epididymal differentiation and sperm maturation.
See full target information NICOL1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38625739

2024

The secreted micropeptide C4orf48 enhances renal fibrosis via an RNA-binding mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Yang,Hongjie Zhuang,Jinhua Li,Ana B Nunez-Nescolarde,Ning Luo,Huiting Chen,Andy Li,Xinli Qu,Qing Wang,Jinjin Fan,Xiaoyan Bai,Zhiming Ye,Bing Gu,Yue Meng,Xingyuan Zhang,Di Wu,Youyang Sia,Xiaoyun Jiang,Wei Chen,Alexander N Combes,David J Nikolic-Paterson,Xueqing Yu

Biological & pharmaceutical bulletin 45:200-206 PubMed35110507

2022

Functional Domain Mapping of Werner Interacting Protein 1 (WRNIP1).

Applications

Unspecified application

Species

Unspecified reactive species

Akari Yoshimura,Tatsuya Sakakihara,Takemi Enomoto,Masayuki Seki

Nature communications 11:1312 PubMed32161263

2020

Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Zhang,Boris Reljić,Chao Liang,Baptiste Kerouanton,Joel Celio Francisco,Jih Hou Peh,Camille Mary,Narendra Suhas Jagannathan,Volodimir Olexiouk,Claire Tang,Gio Fidelito,Srikanth Nama,Ruey-Kuang Cheng,Caroline Lei Wee,Loo Chien Wang,Paula Duek Roggli,Prabha Sampath,Lydie Lane,Enrico Petretto,Radoslaw M Sobota,Suresh Jesuthasan,Lisa Tucker-Kellogg,Bruno Reversade,Gerben Menschaert,Lei Sun,David A Stroud,Lena Ho
View all publications
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Product promise

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