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AB66854

Anti-C5 antibody

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(3 Publications)

Rabbit Polyclonal C5 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

1 Images
Western blot - Anti-C5 antibody (AB66854)
  • WB

Unknown

Western blot - Anti-C5 antibody (AB66854)

All lanes:

Western blot - Anti-C5 antibody (ab66854) at 1 µg/mL

All lanes:

Human Plasma Total Protein Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 188 kDa

Observed band size: 180 kDa,238 kDa,68 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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  • 0
  • 0

Complementary Products

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  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.
Biological function summary

The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.

Pathways

The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.

C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).
See full target information C5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum 11:e0449322 PubMed37191558

2023

Plasmodium falciparum Gametes and Sporozoites Hijack Plasmin and Factor H To Evade Host Complement Killing.

Applications

Unspecified application

Species

Unspecified reactive species

Medard Ernest,Thiago F A Rosa,Zarna Rajeshkumar Pala,Heather M Kudyba,Brendan Sweeney,Timo Reiss,Gabriele Pradel,Joel Vega-Rodríguez

Journal of cellular physiology 236:5012-5021 PubMed33400289

2021

Uromodulin aggravates renal tubulointerstitial injury through activation of the complement pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Fei Pei,Qiaoling Sun,Fei Shen,Xiangdong Yang,Zhao Hu,Maojing Liu

BMC cancer 19:201 PubMed30841875

2019

The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhao,Jun Wu,Feiteng Lu,Xuan Peng,Chenlin Liu,Nanjin Zhou,Muying Ying
View all publications
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