AB97465

Anti-C5 antibody

Name: Anti-C5 antibody (ab97465) | Abcam
Brand: Abcam Limited
SKU: AB97465

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(1 Publication)

Rabbit Polyclonal C5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human C5 aa 1200-1450.

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

1 Images

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Western blot - Anti-C5 antibody (AB97465)

5% SDS-PAGE.

All lanes:

Western blot - Anti-C5 antibody (ab97465) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 188 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human C5 aa 1200-1450. The exact immunogen used to generate this antibody is proprietary information.

P01031

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5 also known as complement 5 is a protein with a mass of approximately 190 kDa. This protein plays a mechanical role as part of the complement system within the immune response. C5 is expressed in the bloodstream and is produced mainly by the liver. Upon activation it splits into C5a and C5b where C5a acts as a potent anaphylatoxin while C5b initiates the assembly of the membrane attack complex. Due to its important functions the production and regulation of C5 are tightly controlled within the body.

Biological function summary

The activation of this target contributes significantly to inflammatory processes and host defense. It is part of the larger complement system where it aligns with other complement proteins like C3 and forms a pathway important for immune system functioning. C5 is integral to the terminal complement complex formation which results in the lysis of target cells. The cleavage product C5a has near-instant effects as a chemotactic agent recruiting other immune cells to sites of infection.

Pathways

The protein has a fundamental position in the classical and alternative complement pathways. These pathways converge at the level of C5 where its cleavage is necessary for further downstream effects. C5 interacts extensively with C3 in these pathways highlighting their joint role in amplifying the immune response. The membrane attack complex which C5 helps initiate is a vital endpoint in these pathways promoting the destruction of pathogen cell membranes.

C5 has been linked to conditions like hereditary angioedema and atypical hemolytic uremic syndrome. Dysregulation of C5 or its pathways can lead to chronic inflammation excessive tissue damage or inefficient pathogen clearance. In such conditions related proteins like C3 also show altered activity patterns. Targeting C5 with specific antibodies has emerged as a therapeutic strategy to manage these diseases aiming to mitigate the pathological activation of the complement system.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

See full target information C5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:15 PubMed31907356

2020

MicroRNA-26a/b have protective roles in oral lichen planus.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Du,Ruifang Gao,Yimei Wang,Tivoli Nguyen,Fang Yang,Yongyan Shi,Tianjing Liu,Wang Liao,Ran Li,Fang Zhang,Xuejun Ge,Bin Zhao

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