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AB11876

Anti-C5 / C5a antibody [557]

4

(1 Review)

|

(6 Publications)

Mouse Monoclonal C5 antibody. Suitable for ELISA, WB, FuncS (Neut/Block) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

1 Images
Functional Studies - Anti-C5 / C5a antibody [557] (AB11876)
  • FuncS

Supplier Data

Functional Studies - Anti-C5 / C5a antibody [557] (AB11876)

ab11876 was used as detection antibody in ELISA. Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1 : 50. Positive Control Recombinant C5. Negative Control C3.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

557

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB, ELISA, FuncS (Neut/Block)

applications

Epitope

Ab11876 recognizes an epitope located within the alpha-chain of C5 that corresponds to C5a.

Specificity

This antibody reacts with an epitope on C5 and C5a. The antibody 557 is capable to inhibit the binding of C5a to the C5a receptor through a competitive mechanism, it does not block the cleavage of C5 into C5a and C5b.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
0.2 µm filtered
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5 also known as the C5 protein is an essential component of the complement system. It is a large glycoprotein with a molecular weight of approximately 196 kDa. It circulates in the blood plasma as part of the immune system where it remains inactive until cleaved into two fragments C5a and C5b. The fragment C5a or C5-a acts as a potent anaphylatoxin and is an important mediator in inflammatory responses. C5 is ubiquitously expressed in various tissues but is abundantly found in liver tissue.
Biological function summary

The role of C5 and its cleavage products are significant in the immune response. C5a a powerful chemoattractant promotes recruitment of neutrophils and other inflammatory cells at sites of infection. C5b initiates the formation of the membrane attack complex (MAC) which is important for lysing pathogens. C5a as part of the C5/C5a axis plays a vital role in regulating immune cell activity and modulating inflammatory processes. The protein is part of the terminal complement complex engaging in immune defense mechanisms.

Pathways

C5 is integral to the classical and alternative complement pathways. In both pathways the cleavage of C5 is a critical step that leads to the eventual formation of the MAC. C5 interacts with several proteins including C3 and C6 to execute its functions effectively. The close relationship between C3 activation and C5 conversion highlights the interconnectedness of complement proteins in these pathways ensuring a robust immune response against pathogens.

C5a has been implicated in a variety of inflammatory and autoimmune conditions. Notably C5a is associated with rheumatoid arthritis and systemic lupus erythematosus due to its role in promoting inflammation. The dysregulation of C5a activity often exacerbates these conditions. Additionally the protein is linked with other complement components such as C4 and C6 in the pathology of these diseases suggesting a potential target for therapeutic interventions aiming to modulate complement activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
See full target information C5
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 11:732163 PubMed35087765

2022

A Novel Cleavage Pattern of Complement C5 Induced by Infection the Chlamydial Protease CPAF.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Peng,Jingping Gao,Zihao Hu,Hongbo Zhang,Lingli Tang,Fuyan Wang,Lei Cui,Shanshan Liu,Yujie Zhao,Hong Xu,Xin Su,Xiaojing Feng,Yiyuan Fang,Jianlin Chen

International journal of molecular sciences 22: PubMed34066088

2021

Alternative C3 Complement System: Lipids and Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Maisa Garcia-Arguinzonis,Elisa Diaz-Riera,Esther Peña,Rafael Escate,Oriol Juan-Babot,Pedro Mata,Lina Badimon,Teresa Padro

Journal of experimental & clinical cancer research 39:9 PubMed31928530

2020

Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitao Yuan,Jinning Ye,Zhenguo Liu,Yufeng Ren,Weiling He,Jianbo Xu,Yulong He,Yujie Yuan

Molecular medicine reports 18:2110-2116 PubMed29956782

2018

C5a aggravates dysfunction of the articular cartilage and synovial fluid in rats with knee joint immobilization.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lu,Lin Wang,Jing Yao,Chunxin Wo,Yu Chen

PLoS pathogens 10:e1004306 PubMed25101794

2014

The Vi capsular polysaccharide enables Salmonella enterica serovar typhi to evade microbe-guided neutrophil chemotaxis.

Applications

Unspecified application, Unspecified application

Species

Mouse, Human

Tamding Wangdi,Cheng-Yuk Lee,Alanna M Spees,Chenzhou Yu,Dawn D Kingsbury,Sebastian E Winter,Christine J Hastey,R Paul Wilson,Volkmar Heinrich,Andreas J Bäumler

Immunotechnology : an international journal of immunological engineering 2:115-26 PubMed9373320

1996

Epitope mapping of a C5a neutralizing mAb using a combined approach of phage display, synthetic peptides and site-directed mutagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

A Kola,M Baensch,W Bautsch,M Hennecke,A Klos,M Casaretto,J Köhl
View all publications
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