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AB11879

Anti-C5 / C5b antibody [568]

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(2 Publications)

Mouse Monoclonal C5 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

568

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with an epitope on C5 and on C5b.

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Complementary Products

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Primary Antibodies

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Primary Antibodies

AB55811

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Primary Antibodies

AB252435

Anti-C5a-R antibody [EPR23278-117]

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C5 molecule also known as complement component 5 is an essential part of the complement system which is part of the immune response. This protein has an approximate molecular weight of 190 kDa and is mainly expressed in the liver circulating in plasma. Upon activation C5 is cleaved into C5a and C5b fragments. The C5b fragment plays an important role in forming the membrane attack complex (MAC) by binding to other complement proteins. The cleavage and activation of C5 are necessary for initiating the assembly of the MAC.
Biological function summary

C5 facilitates the destruction of pathogens by forming the membrane attack complex with other complement proteins such as C6 C7 C8 and C9. C5b acts as a binding site for these proteins leading to a cascade that eventually creates a pore in the pathogen's membrane causing cell lysis. This role in the complement system places C5 as an essential player in innate immunity helping the body eliminate bacteria and foreign cells through direct lysis.

Pathways

C5 functions within the complement pathway specifically in the terminal (lytic) pathway. This pathway has two branches: the classical pathway initiated by antibody-antigen complexes and the alternative pathway activated directly on pathogen surfaces. Both pathways lead to the cleavage of C5 by the C5 convertase resulting in engagement of C5b with C6 C7 C8 and C9 to form the MAC. C5 works closely with other complement proteins including C3 and C4 linking the initial phase of activation to the execution of cell lysis.

C5 has significant connections to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). These diseases result from dysregulation of the complement system and can lead to excessive activation of C5 and the formation of MAC causing damage to host cells. Eculizumab a monoclonal antibody targeting C5 has been used in treatment by inhibiting C5 activation and preventing the formation of the MAC. This intervention reduces hemolysis and other symptoms associated with these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).
See full target information C5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40072066

2025

Finerenone Alleviates Over-Activation of Complement C5a-C5aR1 Axis of Macrophages by Regulating G Protein Subunit Alpha i2 to Improve Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Han Li,Zi-Jun Sun,Sydney C W Tang,Ming-Hui Zhao,Min Chen,Dong-Yuan Chang

Developmental cell 55:224-236.e6 PubMed33038333

2020

Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Liu,Quynh P H Nguyen,Rashmi Nanjundappa,Nathalie Delgehyr,Alexandre Megherbi,Regan Doherty,James Thompson,Claire Jackson,Alexandra Albulescu,Yew M Heng,Jane S Lucas,Sharon D Dell,Alice Meunier,Kirk Czymmek,Moe R Mahjoub,Vito Mennella
View all publications
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Product promise

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