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Rabbit Recombinant Monoclonal C5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Western blot - Anti-C5 / C5b antibody [EPR23940-3] (AB275931), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (AB275931), expandable thumbnail
  • Western blot - Anti-C5 / C5b antibody [EPR23940-3] (AB275931), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (AB275931), expandable thumbnail
  • Western blot - Anti-C5 / C5b antibody [EPR23940-3] (AB275931), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

Alternative names

Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, C5

Recommended products

Rabbit Recombinant Monoclonal C5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23940-3

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931), expandable thumbnail

    Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)

    False colour image of Western blot: Anti-C5 / C5b antibody [EPR23940-3] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab275931 was shown to bind specifically to C5 / C5b. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 20 µg

    Lane 2: U-2 OS cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 188 kDa

    Observed band size: 165-180 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (ab275931), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling C5/C5b with ab275931 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on plasma of human breast. The section was incubated with ab275931 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931), expandable thumbnail

    Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23401592, 25387724).

    Exposure time: 48 seconds.

    All lanes: Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931) at 1/1000 dilution

    Lane 1: Mouse plasma at 20 µg

    Lane 2: Rat plasma at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 188 kDa

    Observed band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (ab275931), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling C5/C5b with ab275931 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human liver. The section was incubated with ab275931 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931), expandable thumbnail

    Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 24071736, 23401592, 25387724)

    Exposure time: 59 seconds.

    All lanes: Western blot - Anti-C5 / C5b antibody [EPR23940-3] (ab275931) at 1/1000 dilution

    Lane 1: Human plasma at 10 µg

    Lane 2: Human liver tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 188 kDa

    Observed band size: 190 kDa, 70 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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