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AB11878

Anti-C5a / C5a des Arg antibody [2952]

4

(1 Review)

|

(7 Publications)

Mouse Monoclonal C5 antibody. Suitable for ELISA, WB, IHC-P, FuncS (Neut/Block), IHC-Fr and reacts with Human samples. Cited in 7 publications.

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2952

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-Fr, FuncS (Neut/Block), IHC-P, ELISA, WB

applications

Specificity

This antibody reacts with a neo-epitope (des-Arg) on C5a that is formed upon cleavage of C5 into C5a and C5b. It will detect C5a and C5a des-Arg (Not C5).

Please notice that under given conditions it is known that C5 can expose epitopes normally only found in the cleaved activation products.

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Alternative Products

Primary Antibodies

AB202039

Anti-C5a antibody [EPR19699-24]

RabMAb|Recombinant|Trial Size|Trial Size

Western blot - Anti-C5a antibody [EPR19699-24] (AB202039)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-C3 antibody [EPR19394] (AB200999)

  • 4
  • 9
Primary Antibodies

AB55811

Anti-C5b-9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5b-9 antibody (AB55811)

  • 3
  • 4
Primary Antibodies

AB252435

Anti-C5a-R antibody [EPR23278-117]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [EPR23278-117] (AB252435)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
0.2 µm filtered
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C5a protein also known as anaphylatoxin C5a is a small potent molecule weighing approximately 11 kDa. It originates from the larger complement component C5 during complement system activation. The C5a des Arg is a slightly modified version produced when enzymes cleave the C-terminal arginine from C5a. Both forms express on cell surfaces and recognize specific receptors on target cells particularly in immune and inflammatory responses.
Biological function summary

This protein controls complex interactions within the immune system specifically as part of the cleavage product of C5 a component of the complement system. C5a binds to receptors such as C5aR and C5L2 which appear in high numbers on various immune cells like macrophages neutrophils and endothelial cells. This binding triggers multiple intracellular pathways mediating inflammatory processes and immune cell activation.

Pathways

The complement system involves the activation sequences where C5a plays a pivotal role. It participates in the activation of the classic and the alternative complement pathways contributing to the opsonization and lysis of pathogens. C5a works closely with proteins such as C3a and C9 orchestrating the body's defense mechanisms against infections while facilitating communication between the innate and adaptive immune systems.

C5a plays a notable role in inflammatory diseases like rheumatoid arthritis and sepsis. Its overactivation relates to severe inflammatory responses and tissue damage. The link between C5a and other inflammatory mediators such as tumor necrosis factor-alpha (TNF-alpha) emphasizes its contribution to the pathogenesis of these conditions. Understanding these pathways offers insight into potential therapeutic targets for modulating excessive inflammation and immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).
See full target information C5
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40072066

2025

Finerenone Alleviates Over-Activation of Complement C5a-C5aR1 Axis of Macrophages by Regulating G Protein Subunit Alpha i2 to Improve Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Han Li,Zi-Jun Sun,Sydney C W Tang,Ming-Hui Zhao,Min Chen,Dong-Yuan Chang

JCI insight 7: PubMed36546481

2022

Deficiency of CFB attenuates renal tubulointerstitial damage by inhibiting ceramide synthesis in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Jun Sun,Dong-Yuan Chang,Min Chen,Ming-Hui Zhao

Journal of experimental & clinical cancer research 39:9 PubMed31928530

2020

Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kaitao Yuan,Jinning Ye,Zhenguo Liu,Yufeng Ren,Weiling He,Jianbo Xu,Yulong He,Yujie Yuan

Nature communications 9:4186 PubMed30305631

2018

Complement receptor CD46 co-stimulates optimal human CD8 T cell effector function via fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppina Arbore,Erin E West,Jubayer Rahman,Gaelle Le Friec,Nathalie Niyonzima,Mehdi Pirooznia,Ilker Tunc,Polychronis Pavlidis,Nicholas Powell,Yuesheng Li,Poching Liu,Aude Servais,Lionel Couzi,Veronique Fremeaux-Bacchi,Leo Placais,Alastair Ferraro,Patrick R Walsh,David Kavanagh,Behdad Afzali,Paul Lavender,Helen J Lachmann,Claudia Kemper

Scientific reports 6:29177 PubMed27381421

2016

High Level of Anaphylatoxin C5a Predicts Poor Clinical Outcome in Patients with Clear Cell Renal Cell Carcinoma.

Applications

IHC

Species

Human

Wei Xi,Li Liu,Jiajun Wang,Yu Xia,Qi Bai,Qilai Long,Yiwei Wang,Jiejie Xu,Jianming Guo

Molecular vision 17:2856-63 PubMed22128233

2011

Aqueous cytokine and growth factor levels do not reliably reflect those levels found in the vitreous.

Applications

WB

Species

Human

Stephanie M Ecker,Joshua C Hines,Scott M Pfahler,Bert M Glaser

Proceedings of the National Academy of Sciences of 103:2328-33 PubMed16452172

2006

Drusen complement components C3a and C5a promote choroidal neovascularization.

Applications

IHC-Fr, IHC-Fr

Species

Mouse, Human

Miho Nozaki,Brian J Raisler,Eiji Sakurai,J Vidya Sarma,Scott R Barnum,John D Lambris,Yali Chen,Kang Zhang,Balamurali K Ambati,Judit Z Baffi,Jayakrishna Ambati
View all publications
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Product promise

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