AB303662

Anti-C5a / C5a des Arg antibody [C17/5]

Recombinant
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Mouse Recombinant Monoclonal C5 antibody. Suitable for WB, I-ELISA and reacts with Human, Recombinant full length protein - Human samples.

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

3 Images

Indirect ELISA - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

I-ELISA

Supplier Data

Indirect ELISA - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Indirect ELISA (dose response curve) of antigen Human C5a at a concentration of 5000 ng/ml. Primary antibody ab303662 used at 0-2000 ng/ml. Secondary antibody was a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at 1/1000 dilution.

Supplier Data

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (ab303662) at 1/1000 dilution

Lane 1:

Human C5 Recombinant protein at 40 ng

Lane 2:

Human C5a Recombinant protein at 40 ng

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 10 kDa,110 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature (PMID : 1710957). Very low or no expression of C5 alpha chain is observed in skeletal muscle. 110 kDa Complement C5 alpha chain is observed. Exposure time : 60 seconds

All lanes:

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (ab303662) at 1/1000 dilution

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/5000 dilution

Observed band size: 110 kDa

false

Exposure time: 60s

Carrier free

Anti-C5a / C5a des Arg antibody [C17/5] - BSA and Azide Free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C17/5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C5a protein also known as anaphylatoxin C5a is a small potent molecule weighing approximately 11 kDa. It originates from the larger complement component C5 during complement system activation. The C5a des Arg is a slightly modified version produced when enzymes cleave the C-terminal arginine from C5a. Both forms express on cell surfaces and recognize specific receptors on target cells particularly in immune and inflammatory responses.

Biological function summary

This protein controls complex interactions within the immune system specifically as part of the cleavage product of C5 a component of the complement system. C5a binds to receptors such as C5aR and C5L2 which appear in high numbers on various immune cells like macrophages neutrophils and endothelial cells. This binding triggers multiple intracellular pathways mediating inflammatory processes and immune cell activation.

Pathways

The complement system involves the activation sequences where C5a plays a pivotal role. It participates in the activation of the classic and the alternative complement pathways contributing to the opsonization and lysis of pathogens. C5a works closely with proteins such as C3a and C9 orchestrating the body's defense mechanisms against infections while facilitating communication between the innate and adaptive immune systems.

C5a plays a notable role in inflammatory diseases like rheumatoid arthritis and sepsis. Its overactivation relates to severe inflammatory responses and tissue damage. The link between C5a and other inflammatory mediators such as tumor necrosis factor-alpha (TNF-alpha) emphasizes its contribution to the pathogenesis of these conditions. Understanding these pathways offers insight into potential therapeutic targets for modulating excessive inflammation and immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

See full target information C5

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C5a / C5a des Arg antibody [C17/5]

Indirect ELISA - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise