Anti-C5a-R antibody [10/92]

• Flow Cyt

Lab

Flow Cytometry - Anti-C5a-R antibody [10/92] (AB117579)

Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling C5a-R with ab117579 at 1/50 dilution (0.1ug) / Right compared with a Rat monoclonal IgG / Left isotype control.

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) was used as a secondary at a 1/5000 dilution.

Gated on viable cells.
Cells were co-stained with anti mouse CD11b conjugated to Brilliant Violet 421.

• Flow Cyt

Lab

Flow Cytometry - Anti-C5a-R antibody [10/92] (AB117579)

Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling C5a-R with ab117579 at 1/50 dilution (0.1ug) / Right compared with a Rat monoclonal IgG / Left isotype control.

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) was used as a secondary at a 1/5000 dilution.

Gated on viable cells.
Cells were co-stained with anti mouse CD19 conjugated to Alexa Fluor®647.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [10/92] (AB117579)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells with ab117579 at 1/500 dilution followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor® 594) was used for counterstain at a 1/200 dilution (Magenta).

Confocal image showing membranous and cytoplasmic staining in subsets of mouse PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).