AB234757

Anti-C5a-R antibody

Name: Anti-C5a-R antibody (ab234757) | Abcam
Brand: Abcam Limited
SKU: AB234757
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal C5a-R antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human C5AR1 aa 1-50.

View Alternative Names

CD88, C5AR, C5R1, C5AR1, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody (AB234757)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody (AB234757)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for C5a-R (green) using ab234757 at 1/100 dilution in ICC/IF, followed by Alexa Fluor^® 488 conjugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-C5a-R antibody (AB234757)

All lanes:

Western blot - Anti-C5a-R antibody (ab234757) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000000 dilution

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human C5AR1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P21730

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5a-R also known as CD88 or C5aR1 is a protein found primarily on the surface of immune cells like macrophages and neutrophils. Its mass is approximately 45 kDa. C5a-R functions as a G protein-coupled receptor (GPCR) and binds the complement component C5a a powerful inflammatory peptide. The receptor is expressed in various tissues but primarily in the immune system where it plays a significant role in immune responses and inflammatory processes.

Biological function summary

C5a-R functions as a mediator of inflammation and immune responses. It is a part of the complement system a complex of proteins involved in innate immunity. Upon binding to C5a this receptor initiates a signaling cascade that leads to the recruitment and activation of immune cells at sites of inflammation. It amplifies the inflammatory response leading to cell chemotaxis degranulation and the production of pro-inflammatory cytokines.

Pathways

C5a-R plays an important role in the complement and immune pathways. It interacts closely with the C5 protein which is a core component of the complement pathway. This receptor also connects to other immune system proteins such as the C3 and C4 proteins through the cascade. Together these interactions help defend against pathogenic infections and maintain immune homeostasis.

C5a-R is associated with conditions like rheumatoid arthritis and sepsis. In rheumatoid arthritis the receptor's activation by C5a contributes to chronic inflammation and joint damage. During sepsis overactivation of C5a-R can lead to excessive inflammation and tissue damage. The receptor's involvement in these diseases is often linked with other proteins in the complement system such as C3a and C4a which also play roles in modulating inflammation and immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed : 10636859, PubMed : 15153520, PubMed : 1847994, PubMed : 29300009, PubMed : 7622471, PubMed : 8182049, PubMed : 9553099). The ligand interacts with at least two sites on the receptor : a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed : 7622471, PubMed : 8182049, PubMed : 9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed : 10636859, PubMed : 15153520).

See full target information C5AR1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 195:1158-1171 PubMed40056975

2025

C5aR1 Promotes Invasion, Metastasis, and Poor Prognosis in Cutaneous Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lauri Heiskanen,Liisa Nissinen,Elina Siljamäki,Jaakko S Knuutila,Teijo Pellinen,Markku Kallajoki,Jyrki Heino,Pilvi Riihilä,Veli-Matti Kähäri

The EMBO journal 44:1154-1184 PubMed39753953

2025

A bipartite bacterial virulence factor targets the complement system and neutrophil activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kurni Kurniyati,Nicholas D Clark,Hongxia Wang,Yijie Deng,Ching Wooen Sze,Michelle B Visser,Michael G Malkowski,Chunhao Li

Inflammation 47:1820-1836 PubMed38565760

2024

Mast Cells are Dependent on Glucose Transporter 1 (GLUT1) and GLUT3 for IgE-mediated Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mirjana Grujic,Md Abdul Alim,Lars Hellman,Magnus Peterson,Gunnar Pejler

Experimental and therapeutic medicine 24:493 PubMed35837068

2022

The complement C3a-C3aR and C5a-C5aR pathways promote viability and inflammation of human retinal pigment epithelium cells by targeting NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Luo,Huiyan Xu,Xuechun Gong,Jinyan Shen,Xuan Chen,Zhifeng Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C5a-R antibody

Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody (AB234757)

Western blot - Anti-C5a-R antibody (AB234757)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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