Anti-C5b-9 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5b-9 antibody (AB55811)

ab55811 staining C5b-9 in human liver.
Left panel : with primary antibody at 4 ug/ml. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C5b-9 antibody (AB55811)

ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• IHC-P

AbReview77864****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5b-9 antibody (AB55811)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling C5b-9 with ab55811 at 1/100 dilution (24h at 4°C ) followed by Goat anti Rabbit Alexa Fluor® 488 at 1/1000 dilution (Green).

Red channel corresponds to Evans Blue and white channel is the fluorescence obtained from nuclear counterstain DAPI staining.

Heat mediated antigen retrieval using Bio SB ImmunoRetriever with citrate

This image is courtesy of an Abreview submitted by Marcelo Pelajo Machado

• IHC

CiteAb

Immunohistochemistry - Anti-C5b-9 antibody (AB55811)

C5b-9 immunohistochemistry using Anti-C5b-9 antibody ab55811. Publication image and figure legend from Yang, H., Li, G., et al., 2024, Front Immunol, PubMed 39483473

C5a-C5aR pathway activation in human breast cancer and its role in ferroptosis resistance and breast cancer progression in 4T-1 xenograft mouse model. (A), the expression of C5b-9 and C5aR in BC tumoral tissues (T) and BC-adjacent non-tumoral tissues (N) of patients with different clinical stages were evaluated by immunohistochemistry. Statistical analyses of the difference of C5b-9 and C5aR expression between different groups were presented in the right. Statistical differences are assessed by student’s t-test. Data were mean ± s.e.m. *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.001.