Anti-C5b-9 rabbit polyclonal antibody that is used to detect C5b-9 in IHC, ICC/IF. Suitable for Human samples.
- Recognises C5b-9 complex or complement C5, also known as the membrane attack complex (MAC), which plays a crucial role in the immune system’s defense mechanism
- No reactivity with non-activated normal human serum or plasma
- Cited in over 80 publications
- Trusted since 2007
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Publications
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- Experimental and therapeutic medicine 26:5762023Downregulation of TRPC6 regulates ERK1/2 to prevent sublytic C5b‑9 complement complex‑induced podocyte injury through activating autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Li,Youfu Fang,Jing LiuPubMed 38023364
- Diabetologia 66:1705-17182023Cholesterol crystal formation is a unifying pathogenic mechanism in the development of diabetic retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra S Hammer,Tim F Dorweiler,Delaney McFarland,Yvonne Adu-Agyeiwaah,Natalia Mast,Nicole El-Darzi,Seth D Fortmann,Sunil Nooti,Devendra K Agrawal,Irina A Pikuleva,George S Abela,Maria B Grant,Julia V BusikPubMed 37311879
- Hypertension research : official journal of the Japanese Society of Hypertension 46:1759-17702023Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Wang,Zhe Zhang,Yuan Li,Ying Zhang,Min Wei,Hui Li,Shanzhi Yang,Yali Zhou,Xinjin Zhou,Guolan XingPubMed 37188751
- Pathology, research and practice 247:1545192023Complement and complement regulatory proteins are upregulated in lungs of COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowen Ge,Zhui Yu,Xinxin Guo,Ling Li,Ling Ye,Maosong Ye,Jingping Yuan,Chouwen Zhu,Weiguo Hu,Yingyong HouPubMed 37244049
- Experimental dermatology 32:632-6402023Successful pharmacological intervention at different levels of the complement system in an in vitro complement fixation model for bullous pemphigoid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJenny Giang,Martijn B A van Doorn,Gilles F H Diercks,Santiago Rodriguez de Cordoba,Thierry P P van den Bosch,Marco W J Schreurs,Felix Poppelaars,Jeffrey DammanPubMed 36704908
- Nature communications 14:4732023The classical pathway triggers pathogenic complement activation in membranous nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLarissa Seifert,Gunther Zahner,Catherine Meyer-Schwesinger,Naemi Hickstein,Silke Dehde,Sonia Wulf,Sarah M S Köllner,Renke Lucas,Dominik Kylies,Sarah Froembling,Stephanie Zielinski,Oliver Kretz,Anna Borodovsky,Sergey Biniaminov,Yanyan Wang,Hong Cheng,Friedrich Koch-Nolte,Peter F Zipfel,Helmut Hopfer,Victor G Puelles,Ulf Panzer,Tobias B Huber,Thorsten Wiech,Nicola M TomasPubMed 36709213
- Cell death & disease 13:10312022Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Sun,Yajie Ni,Qingmiao Lu,Yan Liang,Mengru Gu,Xian Xue,Chunsun DaiPubMed 36494334
- Neuropathology : official journal of the Japanese Society of Neuropathology 43:252-2562022Macroglossia in rapidly progressive inclusion body myositis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshimune Yamasaki,Akihiro Mukaino,Satoshi Yamashita,Yousuke Takeuchi,Nozomu Tawara,Ryoji Yoshida,Yumi Honda,Taro Yamashita,Asako Kakimoto,Hidetsugu Ueyama,Yukio AndoPubMed 36349419
- Virologica Sinica 38:66-742022Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Yang,Chen Zhang,Yan-Hua Wu,Li-Bo Liu,Zi-Da Zhen,Dong-Ying Fan,Zheng-Ran Song,Jia-Tong Chang,Pei-Gang Wang,Jing AnPubMed 36241087
- Translational research : the journal of laboratory and clinical medicine 249:1-122022MASP-2 and MASP-3 inhibitors block complement activation, inflammation, and microvascular stasis in a murine model of vaso-occlusion in sickle cell disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn D Belcher,Julia Nguyen,Chunsheng Chen,Fuad Abdulla,Ruan Conglin,Zalaya K Ivy,Jason Cummings,Thomas Dudler,Gregory M VercellottiPubMed 35878790
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: 99.9% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Native Full Length Protein corresponding to Human C5. Database link P01031
Consider this alternative
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Alternative names
CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Recommended products
Anti-C5b-9 rabbit polyclonal antibody that is used to detect C5b-9 in IHC, ICC/IF. Suitable for Human samples.
- Recognises C5b-9 complex or complement C5, also known as the membrane attack complex (MAC), which plays a crucial role in the immune system’s defense mechanism
- No reactivity with non-activated normal human serum or plasma
- Cited in over 80 publications
- Trusted since 2007
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: 99.9% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Native Full Length Protein corresponding to Human C5. Database link P01031
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma
- Concentration
- Purification notes
This antibody is provided as IgG fraction. Please note that the concentration value refers to the fraction.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-C5b-9 antibody (ab55811) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P.
Anti-C5b-9 antibody (ab55811) has been cited over 82 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-C5b-9 antibody (ab55811) has high sensitivity and specificity.
Anti-C5b-9 antibody (ab55811) has 4 independent reviews from customers.
Anti-C5b-9 antibody (ab55811) specifically detects C5b-9 (UniProt ID: P13671; Molecular weight: 102kDa) and is sold in 100 µg selling sizes. The C5b-9 complement complex is a membrane attack complex that's part of the body's innate and acquired immune system and is formed when the complement system is activated.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C5b-9 also known as the membrane attack complex (MAC) serves as a critical component in the immune system's defense mechanism. Composed of proteins C5b C6 C7 C8 and multiple units of C9 this complex forms on the surface of pathogen membranes. It typically weighs approximately 900 kDa. The MAC largely expresses on cell membranes during immune responses and primarily targets bacterial pathogens by creating pores that lead to cell lysis.
- Biological function summary
C5b-9 functions as part of the larger complement system which aids in innate immunity. The process begins once C5b initiates the assembly of the complex by binding to C6 C7 C8 and finally incorporating C9 molecules. These form a pore-like structure leading to increased permeability of the target cell membrane resulting in the disruption and elimination of the pathogen.
- Pathways
C5b-9 plays a critical role in the complement cascade an essential pathway in the immune system. This cascade consists of several steps including the alternative and classical pathways. C5b-9 is related to the protein C3 through these pathways which upon activation helps facilitate C5 activation. This connection highlights its importance in promoting pathogen clearance.
- Associated diseases and disorders
C5b-9 has associations with conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). In PNH excessive formation of this complex on red blood cells leads to their destruction contributing to hemolysis. Similarly in aHUS dysregulation of the complement system including C5b-9 causes damage to endothelial cells. This complex connects with proteins like factor H and CD59 whose dysfunction or deficiency can lead to or exacerbate these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5b-9 antibody (ab55811)
ab55811 staining C5b-9 in human liver.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-C5b-9 antibody (ab55811)
ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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