AB66768

Anti-C5b-9 + C5b-8 antibody [aE11]

Name: Anti-C5b-9 + C5b-8 antibody [aE11], mouse monoclonal (ab66768) | Abcam
Brand: Abcam Limited
SKU: AB66768
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(40 Publications)

Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768) is a mouse monoclonal antibody detecting C5b-8 in Flow Cytometry, IHC-Fr, ICC/IF, ELISA. Suitable for Baboon, Horse, Human, Monkey, Pig.

- Over 30 publications
- Trusted since 2008

View Alternative Names

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement component C7, C7, Complement component C8 alpha chain, Complement component 8 subunit alpha, C8A, Complement component C9, C9, Complement component C6, C6

3 Images

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

Immunohistochemical analysis of frozen tonsil tissue section labeling C5b-9 + C5b-8 with ab66768 at a dilution of 1/10.

Vascular endothelia and dendritic cells of germinal center have strong and intensive label.

IHC-Fr

AbReview42976****

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

ab66768 staining C5b-9 + C5b-8 in monkey retina cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor^® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification : 20X.

This image is courtesy of an anonymous Abreview

IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

ab66768 staining C5b-9 + C5b-8 (red) in porcine liver tissue by Immunohistochemistry (Frozen sections).

Tissue was taken from allotransplants of wild-type or a1,3-galactosyltransferase gene-knockout (GTKO) pig liver, 2 hours after grafting or at necroscopy (nec). Tissue was fixed with paraformaldehyde and blocked with 20% donkey serum for 1 hour at room temperature, before being incubated with primary antibody (1/100 in diluent).

Image from Ekser B et al., PLoS One. 2012;7(1):e29720. Epub 2012 Jan 11. Fig 3.; doi:10.1371/journal.pone.0029720; January 11, 2012, PLoS ONE 7(1): e29720.

Biotin

Biotin Anti-C5b-9 + C5b-8 antibody [aE11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

aE11

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human, Baboon, Monkey, Pig, Horse

Applications

ICC/IF, Flow Cyt, IHC-Fr, ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human C9.

P02748

Specificity

The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).

ab66768 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive) (Mollnes TE, Harboe M (1987).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/10 - 1/500", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Baboon": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/10 - 1/500", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/10 - 1/500", "IHCFr-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/10 - 1/500", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Product Specifications
Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt, ICC/IF, IHC-Fr in baboon, horse, human, monkey, pig samples.
Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768) specifically detects C5b-9 +C5b-8 (UniProt ID: P02748; Molecular weight: 61kDa) and is sold in 100 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768) has high sensitivity and specificity.
Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768) has been cited over 33 times in peer reviewed journals and is trusted by the scientific community.

Related Products
Conjugation-ready, carrier free format available for antibody clone aE11 - ab271877.
Antibody clone aE11 is also available pre-conjugated to a variety of labels for your convenience - Biotin (ab237699).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: Phosphate Buffer, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Store undiluted

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The C5b-9 complex also known as the membrane attack complex (MAC) plays an important role in the immune system specifically in the complement cascade. This complex forms as a result of the sequential interaction of complement proteins C5b C6 C7 C8 and multiple C9 molecules creating a pore in the membrane of target cells leading to cell lysis. The C5b-8 serves as an intermediary in the formation of the C5b-9 complex and contributes to the initial assembly of the membrane attack complex. Expression of these components predominantly occurs on the surface of lymphocytes and activated macrophages though the exact mass of the assembled complex is less often quoted in standard references.

Biological function summary

C5b-9 disrupts cellular homeostasis by forming transmembrane channels that permit unregulated ion movement. This function is pivotal in innate immunity where it eliminates virally infected and neoplastic cells. The C5b-9 complex forms through progressive recruitment and insertion of complement proteins into pathogenic membranes with each molecule one step closer to full assembly and execution of its lytic activity. In many immune system-related literature it represents terminal complement complexation signifying the endpoint of complement-mediated cytotoxicity.

Pathways

C5b-9 is an integral component of the complement system's terminal pathway. This pathway escalates from the activation of C3 to the cleavage of C5 with C5b serving as an anchor for further protein assembly. The complement cascade also intersects with coagulation pathways bridging roles between immune defense and clotting functions. In this pathway association with proteins such as C3b and C6 is essential for initiating and propagating complex formation and ultimately cellular destruction.

The C5b-9 complex has notable involvement in autoimmune diseases and renal disorders. Paroxysmal nocturnal hemoglobinuria a condition characterized by red blood cell lysis directly involves excessive C5b-9 deposition due to defective complement regulation on host cells. Similarly atypical hemolytic uremic syndrome which results in kidney damage often links to mutations affecting regulators of C5b-9 activity showing the complex's contribution to pathogenesis. Employing therapeutic strategies researchers often target components like C5 and C9 to mitigate the harmful overactivation or misdirection of this complement activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).

See full target information C5

Additional targets

C9,C6,C7,C8A

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Kidney international reports 9:3145-3155 PubMed39534192

2024

SARS-CoV-2 Protein Deposition Enhances Renal Complement Activation and Aggravates Kidney Injury in Membranous Nephropathy After COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Guoqin Wang,Lei Yang,Xiaoyi Xu,Weiyi Guo,Lijun Sun,Yanyan Wang,Wenrong Cheng,Nan Ye,Lingqiang Kong,Xiaoyi Zhao,Hong Cheng

American journal of nephrology 55:345-360 PubMed38330925

2024

Klotho Stabilizes the Podocyte Actin Cytoskeleton in Idiopathic Membranous Nephropathy through Regulating the TRPC6/CatL Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Wang,Hongyan Liu,Hong Cheng,Xue Xue,Yamei Ge,Xiaoqin Wang,Jun Yuan

Virulence 15:2306691 PubMed38251716

2024

HylS', a fragment of truncated hyaluronidase of , contributes to immune evasion by interaction with host complement factor C3b.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Xu,Long Chen,Siqi Pang,Qiuhong Zhang,Simin Deng,Jiaqi Zhu,Xiabing Chen,Paul R Langford,Qi Huang,Rui Zhou,Lu Li

Med (New York, N.Y.) 4:761-777.e8 PubMed37863058

2023

Shiga toxin targets the podocyte causing hemolytic uremic syndrome through endothelial complement activation.

Applications

Unspecified application

Species

Unspecified reactive species

Emily E Bowen,Jennifer A Hurcombe,Fern Barrington,Lindsay S Keir,Louise K Farmer,Matthew D Wherlock,Carolina G Ortiz-Sandoval,Valentina Bruno,Arlette Bohorquez-Hernandez,Daniel Diatlov,Niyousha Rostam-Shirazi,Sara Wells,Michelle Stewart,Lydia Teboul,Abigail C Lay,Matthew J Butler,Robert J P Pope,Eva M S Larkai,B Paul Morgan,John Moppett,Simon C Satchell,Gavin I Welsh,Patrick D Walker,Christoph Licht,Moin A Saleem,Richard J M Coward

PLoS pathogens 19:e1011023 PubMed36696456

2023

Genome-wide screen in human plasma identifies multifaceted complement evasion of Pseudomonas aeruginosa.

Applications

Unspecified application

Species

Unspecified reactive species

Manon Janet-Maitre,Stéphane Pont,Frerich M Masson,Serena Sleiman,Julian Trouillon,Mylène Robert-Genthon,Benoît Gallet,Chantal Dumestre-Perard,Sylvie Elsen,Christine Moriscot,Bart W Bardoel,Suzan H M Rooijakkers,François Cretin,Ina Attrée

Child neurology open 9:2329048X221140298 PubMed36419651

2022

Miyoshi Muscular Dystrophy Due to Novel Splice Site Variants in Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Bryant,Steven A Moore,James S Nix,Grace Rice,Murat Gokden,Aravindhan Veerapandiyan

Frontiers in immunology 13:918551 PubMed36248901

2022

Complement membrane attack complex is an immunometabolic regulator of NLRP3 activation and IL-18 secretion in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Gisela Jimenez-Duran,Joseph Kozole,Rachel Peltier-Heap,Eleanor R Dickinson,Christopher R Kwiatkowski,Francesca Zappacosta,Roland S Annan,Nicholas W Galwey,Eva-Maria Nichols,Louise K Modis,Martha Triantafilou,Kathy Triantafilou,Lee M Booty

eLife 11: PubMed35748863

2022

Cd59 and inflammation regulate Schwann cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Ashtyn T Wiltbank,Emma R Steinson,Stacey J Criswell,Melanie Piller,Sarah Kucenas

Arthritis & rheumatology (Hoboken, N.J.) 74:1048-1058 PubMed35077006

2022

Contribution of Necroptosis to Myofiber Death in Idiopathic Inflammatory Myopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Lin Peng,Ya-Mei Zhang,Yan-Chun Liu,Lin Liang,Wen-Li Li,Xiao-Lan Tian,Lu Zhang,Hong-Xia Yang,Xin Lu,Guo-Chun Wang

The Journal of pathology 257:314-326 PubMed35239183

2022

Increased cell stiffness contributes to complement-mediated injury of choroidal endothelial cells in a monkey model of early age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea P Cabrera,Jonathan Stoddard,Irene Santiago Tierno,Nikolaos Matisioudis,Mahesh Agarwal,Lauren Renner,Neha Palegar,Martha Neuringer,Trevor McGill,Kaustabh Ghosh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C5b-9 + C5b-8 antibody [aE11]

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (40)

Product promise