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Mouse Monoclonal C5 antibody. Suitable for IHC-Fr, ELISA, Flow Cyt, ICC/IF and reacts with Pig, Monkey, Human, Horse, Baboon samples. Cited in 33 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human C9.
Alternative names=Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, C5
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: Phosphate Buffer, 0.87% Sodium chloride- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Native Full Length Protein corresponding to Human C9. Database link: P02748
Reactivity data
Select an application| WB | IHC-Fr | ELISA | Flow Cyt | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Expected | Expected | Expected |
| Baboon | Not recommended | Predicted | Expected | Expected | Predicted |
| Horse | Not recommended | Expected | Expected | Expected | Expected |
| Monkey | Not recommended | Tested | Predicted | Predicted | Predicted |
| Pig | Not recommended | Tested | Expected | Expected | Predicted |
Images
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Baboon, Horse, Pig, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 1/10.00000 - 1/500.00000 | Notes - |
Species Monkey | Dilution info 1/10.00000 - 1/500.00000 | Notes - |
Species Human | Dilution info 1/10.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Horse | Dilution info 1/10.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Baboon | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Horse, Baboon, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Baboon | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Horse | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Horse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Baboon, Monkey, Pig | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal C5 antibody. Suitable for IHC-Fr, ELISA, Flow Cyt, ICC/IF and reacts with Pig, Monkey, Human, Horse, Baboon samples. Cited in 33 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human C9.
Alternative names=Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, C5
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: Phosphate Buffer, 0.87% Sodium chloride- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Native Full Length Protein corresponding to Human C9. Database link: P02748
- Clone number
aE11
- Purification technique
Affinity purification Protein A/G
- Specificity
The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).
ab66768 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive) (Mollnes TE, Harboe M (1987).
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
Up to 12 months
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Store undiluted
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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